The objectives of the present study were to monitor the effect of backcrossing through microsatellite markers and to compare different marker assisted selection strategies. Four populations were developed using donor parents resistant to the bean golden mosaic virus and, for all crosses, only individuals resistant to the bean golden mosaic virus were backcrossed. For crosses ARC100-4 x DOR303 a...

Bu çal??mada, domateste baz? hastal?k ve zararl?lar için geli?tirilen SCAR CAPS markörlerinin moleküler markör destekli seleksiyon çal??malar?nda geçerlili?ini kontrol etmek amaçlanm??t?r. amaçla Domates lekeli solgunluk virüsü (TSWV), Fusarium solgunlu?u (FOL), yaprak k?v?rc?kl??? (TYLCV), kök-ur nematodu (RKN), gibi zararl?lara dayan?kl?l?k geli?tirilmi? olan markörler test edilmi?tir. markör...

Genotyping is a critical step for molecular marker-assisted selection in rice. Rice genomic DNA samples for genotyping are typically isolated from living tissues such as seedlings. This requires the germination of all candidate seeds and extraction of DNA from the seedlings. Currently, an ideal individual is selected from a very large number of plants, which is time- and labor-consuming, requir...

Gene identification is the process of identifying genomic DNA regions that encode proteins. It has a wide range applications in structural genomics, functional metabolomics, transcriptomics, proteomics, and other genetic-related studies such as genetic disorder detection, treatment prevention. There are several methods for finding genes, including ab-initio method (intrinsic) sequence similarit...