Staphylococcus aureus is a major etiological agent of mastitis in ruminants. We report here the genome sequence of bovine strain Newbould 305, isolated in the 1950s in a case of bovine mastitis and now used as a model strain able to reproducibly induce chronic mastitis in cows.

milk samples were collected from quarters of 35 cows with subclinical mastitis (california mastitis test [cmt] positive and somatic cell counts [scc] >500,000 cells/ml in individual quarter foremilk), as well as from 37 healthy controls. compared to the levels observed in milk from healthy quarters, milk from quarters with subclinical mastitis showed elevated sodium (91.97 vs 52.93 mg/dl), chlo...

Staphylococcus aureus mastitis in dairy sheep ranges from subclinical mastitis to lethal gangrenous mastitis. Neither the S. aureus virulence factors nor the host-factors or the epidemiological events contributing to the different outcomes are known. In a field study in a dairy sheep farm over 21 months, 16 natural isolates of S. aureus were collected from six subclinical mastitis cases, one le...

Factors related to the distribution of clinical bovine mastitis between rear and front quarters were studied using data from a 4 year survey of commercial dairy herds in western France. The study involved 844 mastitis cases affecting 597 lactations of 500 French Friesian cows from 44 herds. Risk factor hypotheses were related to certain aspects of lactation, udder conformation and management pr...

In more than 30% of milk samples from clinical and subclinical bovine mastitis, bacteria fail to grow even after 48 h of conventional culture. The "no-growth" samples are problematic for mastitis laboratories, veterinarians, and dairy producers. This study provides the first investigation of the bacteriological etiology of such samples, using a real-time PCR-based commercial reagent kit. The as...

Protection of the mammary gland against mastitis-causing pathogens is mediated by many factors in thegland and blood circulation. The professional phagocytic cells of bovine udder, polymorphonuclearneutrophils (PMN) and macrophages, comprise the first line of defense against invading mastitis pathogens.Most researchers now accept that the PMN is a key factor in the cows’ defense against intrama...

Staphylococci are the main aetiological factor of bovine mastitis in many dairy herds. Traditionally, coagulase-negative staphylococci (CNS) were considered a normal skin microbiota. Lately the role of CNS in bovine mastitis has increased, as in some countries these pathogens had started to outnumber other mastitis aetiological factors. Given the scale of mastitis problems, the intensive use of...

Introduction On many dairy farms, reproductive failure and the occurrence of mastitis are two of the most common management problems. Risk factors for mastitis and reproductive disorders are similar and it can be difficult to determine the specific impact of mastitis on reproductive performance. Age of cow, heat stress, occurrence of metabolic diseases, immune suppression in early lactation and...

Lactation mastitis is a common, but poorly understood, inflammatory breast disease that is a significant health burden. A better understanding of the aetiology of mastitis is urgently required, and will assist in the development of improved prevention and treatment strategies in both human and animal species. Studies in mice have the potential to greatly assist in identifying new drug candidate...

Infectious mastitis is a common condition among lactating women, with staphylococci and streptococci being the main aetiological agents. In this context, some lactobacilli strains isolated from breast milk appear to be particularly effective for treating mastitis and, therefore, constitute an attractive alternative to antibiotherapy. A (1)H NMR-based metabolomic approach was applied to detect m...