Multivariate curve resolution (MCR) using constrained alternating least squares algorithms represents a powerful analysis capability for the quantitative analysis of hyperspectral image data. We will demonstrate the application of MCR using data from a new hyperspectral fluorescence imaging microarray scanner for monitoring gene expression in cells from thousands of genes on the array. The new ...

3,5-Diamino-1,2,4-triazole (DAT) became a significant energetic materials intermediate, and the study of its reaction mechanism has fundamental significance in chemistry. The aim of this study is to investigate the ability of online attenuated total reflection infrared (ATR-IR) spectroscopy combined with the novel approach of hybrid hard- and soft-modelling multivariate curve resolution-alterna...

Application of multivariate curve resolution alternating least squares (MCR-ALS), for the resolution and quantification of different analytes in different type of pharmaceutical and agricultural samples is shown. In particular, MCR-ALS is applied first to the UV spectrophotometric quantitative analysis of mixtures of commercial steroid drugs, and second to the near-infrared (NIR) spectrophotome...

We studied the presence of the mobile colistin resistance gene mcr-1 in human, animal, and environmental Enterobacteriaceae samples from Cumana, Venezuela, that were collected in 2015. The mcr-1 gene was detected in 2/93 Escherichia coli isolates from swine (novel ST452) and human (ST19) samples that were resistant to colistin. Whole-genome sequencing and transformation experiments identified m...

possible that contaminants in the preparation may have contributed some of the effects observed. The present study describes the purification of MCR to a homogeneous single component as demonstrated by poly acrylamide gel electrophoresis and NH@-terminaIamino acid analysis and presents preliminary information on the ability of MCR to degrade PM2 DNA in vitro and DNA within Novikoff hepatoma cells.

Transmissible colistin resistance conferred by the mcr-1 gene-bearing IncI2 plasmid has been recently reported in Escherichia coli in the United States. We report here the completed genome sequence of a second E. coli strain isolated from swine in the United States that carried the mcr-1 gene on an IncI2-type plasmid.

A novel concept in available bit rate (ABR) service model as de ned by the ATM Forum is the minimum cell rate (MCR) support for each ABR virtual connection. In this paper, we present a fundamental study of rate allocation policies with MCR guarantee. In particular, we de ne three network bandwidth allocation policies that guarantee MCR requirements. For each policy, we present a centralized alg...

Background The emergence of the plasmid-mediated colistin resistance mechanism in Escherichia coli has raised concern among public health experts as colistin is a last-line antimicrobial resort. The primary aim of the study was to investigate the prevalence of this resistance trait in E. coli isolates circulating in the Lombardy region, Northern Italy. The presence of mcr-type genes and their g...

Background: Currently, colistin-resistant pathogens emerged has become a global health concern. This study assessed the distribution of mcr-1 to mcr-5 variants with phenotypic colistin-resistance in bacterial isolates from urinary tract infection (UTI) patients Bangladesh.Methods: A cross-sectional was conducted between April 2017 and March 2018 enroll uncomplicated UTI patients, 142 urine samp...

This paper presents a Multiple Combined Ranker (MCR) approach for answering definitional questions. Generally, our MCR approach first extracts question target-related knowledge as much as possible, then using this knowledge to pick up appropriate question answers. The knowledge includes both online definitions and related terms (RT). In our system, extraction of related terms is different from ...