نتایج جستجو برای: mediated isothermal amplification

تعداد نتایج: 472108  

Journal: : 2022

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has caused worldwide losses from all points of view. Although there are efficient vaccines against this virus, rapid detection remains essential for controlling its spread. gold standard the COVID-19 diagnostic is use quantitative real time polymerase chain reaction (RT-qPCR) naso-/oro-pharyngeal swabs and, alternatively,...

Journal: :BioTechniques 2012
Nathan A Tanner Yinhua Zhang Thomas C Evans

Loop-mediated isothermal amplification (LAMP) is a rapid and reliable sequence-specific isothermal nucleic acid amplification technique. To date, all reported real-time detection methods for LAMP have been restricted to single targets, limiting the utility of this technique. Here, we adapted standard LAMP primers to contain a quencher-fluorophore duplex region that upon strand separation result...

2012
Kelly A. Curtis Donna L. Rudolph Irene Nejad Jered Singleton Andy Beddoe Bernhard Weigl Paul LaBarre S. Michele Owen

BACKGROUND To date, the use of traditional nucleic acid amplification tests (NAAT) for detection of HIV-1 DNA or RNA has been restricted to laboratory settings due to time, equipment, and technical expertise requirements. The availability of a rapid NAAT with applicability for resource-limited or point-of-care (POC) settings would fill a great need in HIV diagnostics, allowing for timely diagno...

Journal: :Revista do Instituto de Medicina Tropical de São Paulo 2016

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