objectives: securinega virosa roxb (ex willd) baill (euphorbaiceae) root bark has been reportedly used in african traditional medicine in the management of mental illnesses. previously, the sleep-inducing potential of the crude methanol root bark of securinega virosa extract and its butanol fraction have been reported. the study aimed to isolate and characterize the bioactive constituent that m...

A reliable, simple and sensitive reversed-phase high-performance liquid chromatographic method was developed for determination of losartan in plasma .Separation is achieved by HPLC after direct injection on a CN (250*4.6 mm) analytical column with a mobile phase composed of sodium hydrogen phosphate buffer acetonitrile tetrahydrofurane methanol and phosphoric acid (0.1:5 :4 :21 :69.9 ) V/V% adj...

The major complement-fixing antigen of Mycoplasma pneumoniae is found in the lipid fraction of the organism. When the lipids of M. pneumoniae were fractionated by column chromatography on silicic acid, serological activity against both rabbit and human immune sera was found in two fractions, B and D. Fraction B, eluted with chloroform-methanol (9:1), was a minor component in terms of total comp...

The pancreas of adult and old mice of various ages has been shown to contain a lipid fraction which is not normally stainable, but which is unmasked by the action of the lipid-solvent methanol: chloroform (1:2 v/v) and hence becomes stainable by the acid haematein reaction. This fraction seems to be contained in the zymogen granules of adult and old mice, but is absent from the pancreas of tto ...

the antioxidant potential of crude methanol extract (ce) as well as chloroform (cf), ethyl acetate (ef) and n-butanol (nf) soluble fractions of enhydra fluctuans lour, which is widely used in indigenous system of medicine for different purposes, were studied. the antioxidant potential of extract/different fractions were evaluated using different in vitro antioxidant models. in addition, total a...

We investigated the effect of Euphorbia supina extract on the production of cellular reactive oxygen species (ROS) in order to evaluate its antioxidative activity. The H2O2-induced ROS generation was measured with dichlorofluorescein-diacetate (DCFH-DA) assay. The acetone+methylene chloride (A+M) and methanol (MeOH) extracts dose-dependently decreased ROS production induced by H2O2 compared to ...

Prior to preparation of CRM candidate of chloramphenicol in methanol with a concentration of 100 mg/L, two independent methods including mass balance (MB) and quantitative nuclear magnetic resonance (qNMR) were employed to precisely measure the mass fraction of pure chloramphenicol materials. The mass fraction was assigned to be 99.8% with uncertainty of 0.3%. Homogeneity testing and stability ...

Vellozia pusilla Pohl is a species of the botanic family Velloziaceae that occurs in the subtropical regions of South America and, although it lives under conditions of high solar irradiation and low water availability, shows great longevity. The methanol extract of roots, stem and leaf sheaths of this species showed an antitumoral activity through the inhibition of the enzyme Topoisomerase I w...

Artemisinin extracted from Artemisia annua L. is the best medicine with the highest efficiency, the most effective and the lowest toxicity in treating ague nowadays. At present, most studies focus on artemisinin and its derivatives, while the study and report about the other active components are rare. This paper purposed to further discover new indication of Artemisia annua L. connecting with ...

A glycosidically bound fraction was prepared by adsorbing a 80% methanol extract from fresh rhizomes of ginger onto a column of Amberlite XAD-2 resin and successively eluting with ethyl acetate or methanol. Enzymatic hydrolysis of this fraction with an acetone powder prepared from fresh ginger and commercial glycosidase liberated such alcohols as geraniol, 2-heptanol, α-terpineol, nerol, linalo...