Cryptococcus neoformans is the most common microorganism found in cerebrospinal fluid (CSF) cytology and causes life-threatening infections in immunocompromised hosts. Although its cytomorphologic features in conventional smear cytology have been well described, those in liquid-based cytology have rarely been. A 73-year-old woman with diffuse large B-cell lymphoma presented with mental confusio...

AIMS To compare the efficacy of histochemical and immunohistochemical methods in detecting forms of Sporothrix schenckii in tissue. METHODS Thirty five cutaneous biopsy specimens from 27 patients with sporotrichosis were stained by histochemical haematoxylin and eosin, periodic acid Schiff, and Gomori's methenamine silver methods and an immunohistochemical (avidin-biotin complex immunoperoxid...

The distribution of lactoferrin and glycoprotein in human bronchial glands has been studied by electron microscopy using an immunoperoxidase method to stain the former and a periodic acid-chromic acid-silver methenamine sequence for the latter, each applied to ultrathin Epon sections. The distribution of lactoferrin corresponds to that of lysozyme. Lactoferrin and lysozyme are both confined to ...

A 67-year-old man who had been prescribed prednisolone for 20 months consulted our department due to repeated episodes of pneumonia. Computed tomography showed a large cavity in the apex of the right lung (Picture A, arrow). A fungal culture of a sample aspirated from the cavity revealed black-colored colonies (Picture B), which were later confirmed to be Aspergillus niger. A Grocott’s methenam...

A direct fluorescent antibody (DFA) method was applied to sputum or tracheal aspirate from 68 patients with clinical or radiological evidence suggesting Pneumocystis carinii pneumonitis, and to 50 control patients. P. carinii was detected by DFA in specimens from 33 of the 69 clinical cases and 3 of the 50 controls. Specimens of lung from 11 of 33 DFA-positive cases were examined histologically...

A middle aged female presented with symptoms of chronic cough, breathlessness, fatigue and weight loss of 4 months duration. Chest Xray (CXR) was suggestive of bilateral upper lobe mass lesion with a differential diagnosis in favour of malignancy. Contrast enhanced computed tomography (CECT) chest showed collapse of right upper lobe and left upper lobe. Bronchoscopy showed thick gelatinous glob...

1 Mones JM, Saldana MJ, Oldham SA. Diagnosis of Pnewnocysti.s carinii pneumonia. Roentgenographic-pathologic correlates based on fiberoptic bronchoscopy specimens for patients with the acquired immunodeficiency syndrome. Chest 1986; 89:522-26 2 Hartman B, Koss M, Hui A, Baumann W, Athos L, Boylen T Pneumocystis carinii pneumonia in the acquired immunodeficiency syndrome (AIDS). Diagnosis with b...

Pulmonary infections are common in human immunodeficiency virus (HIV) positive persons. Pneumoystis carinii (PC), now named as pneumocystis jiroveci, is the most frequently encountered pathogen. The study, carried out from June 1999 to May 2005, comprised as many as 1101 HIV positive persons presumed to be suffering from pneumocystis carinii pneumonia. Induced sputum samples were obtained from ...

Fig. 1. (A) Peripheral blood shows neutrophils with toxic granules and two intra-cytoplasmic yeast forms of Histoplasma capsulatum in the cell on the left (May-Grunwald Giemsa, ×1,000). Inset: The fungus was brightly positive for periodic acid–Schiff stain (hematoxylin counterstain, ×1,000). (B) Hemodilute bone marrow smears showed dysgranulopoiesis with 5% blasts (May-Grunwald Giemsa, ×1,000)....