The slide agglutination test MRSA-Screen (Denka Seiken Co., Niigata, Japan) was compared with the mecA PCR ("gold standard") for the detection of methicillin resistance in Staphylococcus aureus. The MRSA-Screen test detected the penicillin-binding protein 2a (PBP2a) antigen in 87 of 90 genetically diverse methicillin-resistant S. aureus (MRSA) stock culture strains, leading to a sensitivity of ...

Recent reports indicate that community-acquired methicillin-resistant Staphylococcus aureus (MRSA) infections are increasing and may now involve persons without risk factors predisposing for acquisition. To estimate the extent of community MRSA in New York City, the prevalence of S. aureus and MRSA nasal colonization in a well-patient population of 500 children and guardians was determined. The...

Staphylococcal infections are a group of infections caused by the bacterium Staphylococcus but most infections are caused by a type called Staphylococcus aureus. Bacteria are constantly evolving because their genes are constantly changing. The result of this is that some of the bacteria develop more resistance to a certain antibiotic than others. Methicillin-resistant Staphylococcus aureus is a...

In the present study, the effectiveness of a rapid and colorimetric nitrate reductase analysis (NRA) method and resazurin microplate assay (REMA) for rapid determination of methicillin resistance among Staphylococcus aureus was investigated. A total of 275 clinical isolates of S. aureus were included in the present study. Among these isolates, 151 had the mecA gene and were resistant to methici...

A wide variety of antimicrobial cationic agents, such as chlorhexidine gluconate, are commonly used in antiseptic preparations in the prevention of nosocomial infections caused by methicillin-resistant Staphylococcus aureus (MRSA). In this study was investigated the activity of chlorhexidine gluconate against MRSA and methicillin-sensitive Staphylococcus aureus (MSSA). The quantitative suspensi...

The use of antibiotics in farm management (growing crops and raising animals) has become a major area of concern. Its implications is the consequent emergence of antibiotic resistant bacteria (ARB) and accordingly their access into the human food chain with passage of antibiotic resistance genes (ARG) to the normal human intestinal microbiota and hence to other pathogenic bacteria causative hum...

Staphylococcus aureus, or methicillin-resistant S. aureus (MRSA), is a significant pathogen in both nosocomial and community infections. Community-associated MRSA (CA-MRSA) strains tend to be multi-drug resistant and to invade hospital settings. This study aimed to assess the antimicrobial resistance and molecular characteristicsof nasal S. aureus among newlyadmitted inpatients.In the present s...

Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) is mainly associated with swine and is capable of causing zoonotic infections. The methicillin-resistant S. aureus (MRSA) multilocus sequence type (ST) 398 of swine origin is predominant in Europe and North America, whereas ST9 is predominant in Asia. To evaluate the possible emergence of MRSA in swine, we examined the S...

A screening method for methicillin-resistant Staphylococcus aureus (MRSA) by using selective broth and real-time PCR (broth-PCR) was developed and evaluated. The samples (n = 304) were cultured in the broth overnight, followed by nuc gene detection by real-time PCR. nuc-negative samples were further checked for the presence of nuc amplification inhibitors by a PCR internal inhibitor assay. nuc-...

The incidence of nosocomial infections with methicillin-resistant Staphylococcus aureus is of great concern in Japan and the developed world as a whole. Simple typing techniques like coagulase and phage typing are quick and useful for monitoring and evaluating these organisms. In view of this, the current status of antimicrobial susceptibility in Staphylococcus aureus (S. aureus) isolates in Ok...