in recent years of experience fetal hemoglobin (hbf) induction considers as novel therapeutic approach for β-thalassemia and sickle cell in therapeutic approaches. several pharmacologic agents such as hydroxyurea, azacytidine, butyrate deriviates and immunomodulator drugs like pomalidomide and lnalidomide could able to up-regulate hbf level with different molecular and epigenetic paterns (1-4)....

Histone methylation patterns in the human genome, especially in euchromatin regions, have not been systematically characterized. In this study, we examined the profile of histone H3 methylation (Me) patterns at different lysines (Ks) in the coding regions of human genes by genome-wide location analyses by using chromatin immunoprecipitation linked to cDNA arrays. Specifically, we compared H3-KM...

RNA-directed DNA methylation (RdDM) is a nuclear pathway which is comprised of multiple main and accessory protein components, including two plant-specific DNA-dependent RNA polymerases, Pol IV and Pol V, and argonaute (AGO) proteins. Regulation in the RdDM pathway can be achieved via multiple mechanisms, including the spatial distribution of different RdDM components. Here we describe a protoc...

Histone lysine methylation plays a critical role in epigenetic regulation of eukaryotes. To date, studies have shown that lysine residues of K4, K9, K27, K36 and K79 in histone H3 and K20 in histone H4 can be modified by histone methyltransferases (HMTs). Such histone methylation can specifically activate or repress the transcriptional activity to play a key role in gene expression/regulation a...

asthma is a complex, heterogeneous and chronic airway inflammatory disease with different clinical phenotypes caused by diverse triggers and pathophysiological mechanisms. asthma heritability has been established in many genetic studies but it is evident that only genetic elements are not responsible for the development of asthma. increasing rate of asthma incidence during past decades has impl...

breast cancer is the most important and prevalent cancer in women that can be managed if detected in the early stages. recently, there has been much focus on the use of epigenetic changes such as dna methylation in early detection of cancer. as recent research has put forth dok7 cpg island methylation as an epimarker, in the present study, using a quantification method, a cohort of iranian spor...

Objective: Methylation status of Septin9 (SEPT9) and vimentin (VIM) genes in circulating tumor DNA colorectal cancer (CRC) patients is a promising bio-marker for the early detection CRC. The aim present study was to identify methylation promoter regions SEPT9 VIM cohort Indian with biopsy proven cancer. Material Methods: Forty-five consecutive were recruited. 10 mL venous samples collected from...

Alcohol use disorder (AUD) has a complex, multifactorial etiology involving dysregulation across several brain regions and peripheral organs. Acute chronic alcohol consumption cause epigenetic modifications in these systems, which underlie changes gene expression subsequently, the emergence of pathophysiological phenotypes associated with AUD. One such mechanism is methylation, can occur on DNA...