MOTIVATION It is expected that individual genes have intrinsically different variability in the global expressional trend among them. Thus, the consideration of gene-specific expressional properties will help us to distinguish target-selective gene expression over non-selective over-expression. RESULTS The re-standardization and integration of heterogeneous microarray datasets, available from...

This paper introduces a novel approach to gene selection based on a substantial modification of analytic hierarchy process (AHP). The modified AHP systematically integrates outcomes of individual filter methods to select the most informative genes for microarray classification. Five individual ranking methods including t-test, entropy, receiver operating characteristic (ROC) curve, Wilcoxon and...

We have recently proposed a rank-based approach as a new microarray data integration method. The rank-based approach, which converts the expression value of each sample into a rank value within the sample, enables us to directly integrate samples generated by different laboratories and microarray technologies. In this study, we show that a non-parametric scoring method can be efficiently employ...

Differential display and DNA microarray have emerged as the two most popular methods for gene expression profiling. Here, we developed a multicolor fluorescent differential display (FDD) method that combines the virtues of both differential display in signal amplification and DNA microarray in signal analysis. As in DNA microarray, RNA samples being compared can be labeled with either a red or ...

Found in virtually every organism, glycans are essential molecules that play important roles almost aspect of biology. The composition glycome, the repertoire an organism or a biological sample, is often found altered many diseases, including cancer, infectious metabolic and developmental disorders. Understanding how glycosylation glycomic changes enriches our knowledge mechanisms disease progr...

component-resolved  diagnosis (crd) using microarray technology has recently been introduced  with the  aim to  improve diagnosis of allergy. the  aim of this study was to compare performance of this allergen microarray to those of an established extract-based skin prick testing (spt). 45  patients  with  allergic rhinitis  were studied  (16 children  and  29  adults). spt  to dermatophagoides ...

The BASC system provides tools for the integrated mining and browsing of genetic, genomic and phenotypic data. This public resource hosts information on Brassica species supporting the Multinational Brassica Genome Sequencing Project, and is based upon five distinct modules, ESTDB, Microarray, MarkerQTL, CMap and EnsEMBL. ESTDB hosts expressed gene sequences and related annotation derived from ...

To investigate the combination of cetuximab, capecitabine and radiotherapy in the preoperative treatment of patients with rectal cancer, fourty tumour samples were gathered before treatment (T0), after one dose of cetuximab but before radiotherapy with capecitabine (T1) and at moment of surgery (T2). The tumour and plasma samples were subjected at all timepoints to Affymetrix microarray and Lum...

Prediction of clinical behaviour and treatment for cancers is based on the integration of clinical and pathological parameters. Recent reports have demonstrated that gene expression profiling provides a powerful new approach for determining disease outcome. If clinical and microarray data each contain independent information then it should be possible to combine these datasets to gain more accu...