To decrease the time and cost of processing urine cultures, we devised a critical pathway to identify and perform antibiotic susceptibility tests on commonly isolated microbial pathogens within 6 h of growth detection. The strategy was based on eliminating expensive kits and automated procedures when not required. A pathway utilizing a statistical matrix and three rapid biochemical tests requir...

Background The global increase in antibiotic resistance and the high number of implant-associated infections have rendered antibiotics ineffective in certain cases. So it’s time for a new therapy. Bacteriophages are obligatory intracellular parasites of bacterial cells and bacteria can be infected by bacteriophages. They have specificity of infection, due to the presence of specific receptors o...

The Fast Antimicrobial Screen Test (FAST) is a simple and quick screening test developed to detect antibiotic and sulfonamide residues in food animal carcasses in slaughter establishments. This microbial inhibition test detects antimicrobials that are allowed to be used in food animals. It has the ability to detect these antimicrobials at or above the allowable limit in carcass kidney fluids in...

The present report describes the usefulness of a microbial caries screening test in a population of children younger than 6 years. Two hundred children presenting to a private pediatric dental office were screened for Streptococcus mutans using a test adapted for the dental office. The numbers of S. mutans colonies were recorded semiquantitatively and carious lesions were assessed clinically an...

Objective: This study, conducted between March 2015 and July 2016, investigated the sensitivity specificity of OV-16 RDT Dried Blood Spot (DBS) Enzyme-Linked Immunosorbent Assay (ELISA) for skin snip microfilaria (MF) detection in Ogun State.
 Methods: Five hundred eighty-eight (588) members 16 first-line communities 8 endemic Local Government Areas (LGAs) provided fingerstick whole blood ...

The response of microbial respiration from soil organic carbon (SOC) decomposition to environmental changes plays a key role in predicting future trends of atmospheric CO2 concentration. However, it remains uncertain whether there is a universal trend in the response of microbial respiration to increased temperature and nutrient addition among different vegetation types. In this study, soils we...

We developed a pyrazinamidase gene DNA-sequencing method to rapidly identify pyrazinamide resistance-causing mutations in GenoLyse-treated, smear-positive sputum specimens. The sensitivity and specificity were 90.9 and 100%, respectively, compared to those of MGIT drug susceptibility testing, after the exclusion of synonymous mutations and nonsynonymous mutations previously associated with susc...