This paper reports preliminary experiments with a cooperative robot system to augment single cell manipulation tasks. The JHU “Steady-Hand” robot configuration for cell manipulation is reported. Stable force control laws for the “Steady Hand” are revisited. Preliminary experiments validating stable insertion of a micropipette in a mouse embryo are detailed along with formulation of vision based...

Ultrasonically induced microstreaming around bubbles of gas have been shown to cause damage to biological materials in vitro at clinical exposure levels. The potential for ultrasonically induced cavitation and microstreaming in mammalian systems is of interest with respect to the safe application of clinical ultrasound. Ultrasonically induced intravascular microstreaming and formation of thromb...

Resting membrane potentials, electrical resistance, and coupling of chick retinal pigment epithelium cells in tissue culture have been measured with micropipette electrodes. Topical application of NaCl, KCl, MgCl2, and CaCl2 produce rapid, reversible changes in the membrane potentials of these cells. CaCl2 uniquely induces a slow hyperpolarizing wave that can lead to barrages of depolarizing ac...

We evaluate the effective Hamiltonian governing, at the optically resolved scale, the elastic properties of micromanipulated membranes. We identify floppy, entropic-tense and stretched-tense regimes, representing different behaviors of the effective-area elasticity of the membrane. The corresponding effective tension depends on the microscopic parameters (total area, bending rigidity) and on th...