Introduction: Methicillin-resistant Staphylococcus aureus (MRSA) is an important pathogen that causes several nosocomial or community-acquired infections. Adhesion to surfaces and subsequent biofilm formation are the major phases of a staphylococcal infection. The aim of this study was to detect the presence of icaABCD genes in clinical isolates of MRSA. Methods: A total of 110 clinical Staphyl...

Background: Nosocomial infections are frequently caused by Klebsiella pneumoniae. The great majority of antibiotics no longer effective against K. goal this study was to see if there a link between antibiotic resistance and the ability produce biofilms in pneumoniae strains isolated from UTI patients Iraq. Methods: A total 85 non-duplicate clinical samples urine, blood, sputum, wound swabs, Cat...

We describe an automated method for the preparation of yeast genomic DNA capable of preparing thousands of DNAs in parallel from a YAC library. Briefly, the protocol involves four steps: (1) Yeast clones are grown in the wells of 96-well microtiter plates with filter (rather than plastic) well-bottoms, which are embedded in solid growth media; (2) These yeast cultures are resuspended and their ...

This study includes 10 samples isolated from a different source. research paper was to the antibacterial activity and antibiofilm of an alcoholic extract Lemon Myrtle (Backhousia citriodora) leaves, against clinical isolates Pseudomonas aerogenoza. Isolates were diagnosed by VITEK-2 compact system (PCR). The capacity produce biofilm tested using Microtiter plate technique (96 wells). results sh...

Current co-transfection protocols use calcium phosphate precipitation for cultured insect (Sf-9) cells followed by multiple agarose overlays to purify recombinant baculoviruses (1). We report a more effective and convenient method that uses the cationic lipid N[1,1-(2,3-dioleyloxy)propoyl]-N,N,N,trimethylammonium chloride, or DOTMA (2, 3), for cotransfections of Sf-9 cells and uses 96-well micr...

New tripodal gem-(bis-phosphonates) uranophiles were discovered by a screening method that allowed for the selection of ligands with strong uranyl-binding properties in a convenient microtiter-plate format. The method is based on competitive uranium binding by using Sulfochlorophenol S as chromogenic chelate. This dye compound was found to present high uranyl complexation properties and allowed...

A new approach using microtiter plate cultivation with on-line measurement of dissolved oxygen (DO) was applied for medium optimization of mammalian cell culture. Applying dynamic liquid phase balance, oxygen uptake rates were calculated from the DO level and used as an indicator for culture viability. The developed method was successfully applied to optimization of the concentration of glucose...