The purification of sterol carrier protein2 (SCP2), purified 1500-fold to homogeneity from the 303,000 x g supernatant (S303) of rat liver, has recently been described (Noland, B. J., Arebalo, R. E., Hansbury, E., and Scallen T. J. (1980) J. Biol. Chem, 255, 4282-4289). Since SCP2 is required for the synthesis of cholesterol by microsomal membranes, it was decided to test the hypothesis that SC...

Takahashi, Masaru, Hirokazu Shiraishi, Yuji Ishibashi, Kristie L. Blade, Paul J. McDermott, Donald R. Menick, Dhandapani Kuppuswamy, and George Cooper IV. Phenotypic consequences of 1-tubulin expression and MAP4 decoration of microtubules in adult cardiocytes. Am J Physiol Heart Circ Physiol 285: H2072–H2083, 2003. First published July 10, 2003; 10.1152/ajpheart.00396. 2003.—In pressure-overloa...

The epothilones (Epos) are a group of natural products isolated from the myxobacterium, Sorangium cellulosum. They have a mechanism of action similar to that of Taxol, i.e., they stabilize microtubules and induce the formation of microtubule bundles in cells. Because they are simpler in structure than Taxol and preserve their activity in P-glycoprotein-expressing cells, they are being studied a...

The Saccharornyces cerevisiae CDC3, CDClO, CDCl I , and CDC12 genes encode a family of homologous proteins that are not closely related to other known proteins [Haarer BK, Ketcham SR, Ford SK, Ashcroft DJ, and Pringle JR (submitted)]. Temperature-sensitive mutants defective in any of these four genes display essentially identical pleiotropic phenotypes that include abnormal cell-wall deposition...

The LATERAL ORGAN BOUNDARIES (LOB) DOMAIN (LBD) gene family encodes plant-specific transcriptional regulators functioning in organ development. In a screen of Arabidopsis (Arabidopsis thaliana) sequence-indexed transferred DNA insertion mutants, we found disruption of the LOB DOMAIN-CONTAINING PROTEIN20 (LBD20) gene led to increased resistance to the root-infecting vascular wilt pathogen Fusari...