A total of 90 Flavobacterium columnare isolates were recovered from predominant wild fish species in the Mobile River, Alabama, USA. Isolates were identified and confirmed by fatty acid methyl ester analysis and specific PCR amplification. Genomovar ascription was performed using 16S-restriction fragment length polymorphism (RFLP) analysis. The majority of genomovar I isolates were recovered fr...

We genotyped 102 Borrelia burgdorferi sensu lato strains isolated from ticks, animals, and patients in 11 provinces in China by PCR-restriction fragment length polymorphism (PCR-RFLP) amplification of 5S (rrf)-23S (rrl) rRNA gene spacer amplicons and multilocus sequence analysis (MLSA). The results showed that Borrelia garinii was the main genotype in China (65/102) and that it was distributed ...

Gut intraepithelial lymphocytes (IEL) contain two independent T cell receptor alpha/beta + T cell populations, with different V beta repertoires. In DBA/2 mice (Mlsa, IE+), the CD4+ and heterodimeric alpha/beta CD8+ thymodependent T cell pool shows the same deletion of V beta 6, 8.1, and 11+ cells as found in peripheral lymphoid organs. In contrast, such deletions are not observed in the pool o...

This paper presents a very low bit rate speech coder based on HMM (Hidden Markov Model). The encoder carries out phoneme recognition, and transmits phoneme indexes, state durations and pitch information to the decoder. In the decoder, phoneme HMMs are concatenated according to the phoneme indexes, and a sequence of mel-cepstral coefficient vectors is generated from the concatenated HMM by using...

Synthesis of listener vocalisations is one of the focused research areas to improve emotionally coloured conversational speech synthesis. To communicate different intentions, a synthesiser should be capable of generating a broad range of vocalisations with different kinds of acoustic properties. However, the data collection for corpus based methods is necessarily limited in acoustic variability...

Pseudomonas strains originating from symptomatic (bacterial spot) leaf tissues of sweet cherry (Topola, Sumadija) and plum (Krusedol Selo, Srem) were isolated during 2016 2020, respectively. Based on the findings yielded by classical microbiological methods, LOPAT (+---+), GATTa (--++) pathogenicity tests performed detached fruitlets (sweet sour cherry) pods (bean pods), all confirmed to belong...