نتایج جستجو برای: molecular cloning
تعداد نتایج: 685841 فیلتر نتایج به سال:
due to the widespread applications of xylitol dehydrogenase, an enzyme used for the production of xylitol, the present study was designed for the cloning of xylitol dehydrogenase gene from glcunobacter oxydans dsm 2003. after extraction of genomic dna from this bacterium, xylitol dehydrogenase gene was replicated using polymerase chain reaction (pcr). the amplified product was entered into ptz5...
The in vitro cloning of DNA molecules traditionally uses PCR amplification or site-specific restriction endonucleases to generate linear DNA inserts with defined termini and requires DNA ligase to covalently join those inserts to vectors with the corresponding ends. We have used the properties of Vaccinia DNA topoisomerase I to develop a ligase-free technology for the covalent joining of DNA fr...
Here, we compare the molecular mechanism of soybean heterosis through the differential expression of basic cloning. Specifically, we cloned 22 differentially expressed cDNA fragments from hybrid combinations of Jilin 38 x EXP (which had obvious yield advantages) and their parents. In addition, we compared the homology of these fragments and predicted their functions. Cloning differentially expr...
KIF1, a new short member of the UNC104/KIF1-Family of Kinesins.
Genetic mapping and positional cloning of genetically complex traits in the laboratory rat (Rattus norvegicus) has recently led to the identification of various susceptibility genes in different rat models. Rat genetics has benefited from revolutionary advances in molecular biology, genetics, genomics and informatics and provide an unparalleled resource for molecular genetic investigation of ma...
Cloning of the maize rough dwarf virus genome: molecular confirmation of the plant-reovirus classification scheme and identification of two large nonoverlapping coding domains within a single genomic segment. Title Cloning of the maize rough dwarf virus genome: molecular confirmation of the plant-reovirus classification scheme and identification of two large nonoverlapping coding domains within...
The importance of the IgE response in protection against infection by schistosomes has been demonstrated in both animal models and in man [1,2]. Among the targets of this response [3], the potential role of a protein of 22 kDa has been emphasized [4] and molecules of 20-22 kDa are widely recognized by IgE in human infection sera (manuscript in preparation). We therefore developed a cloning stra...
Cloning of the maize rough dwarf virus genome: molecular confirmation of the plant-reovirus classification scheme and identification of two large nonoverlapping coding domains within a single genomic segment. Title Cloning of the maize rough dwarf virus genome: molecular confirmation of the plant-reovirus classification scheme and identification of two large nonoverlapping coding domains within...
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