8-Oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) is a commonly formed DNA lesion that is useful as a biomarker for oxidative stress. Although methods for selective quantification of 8-oxodGuo exist, there is room for additional methods that are sensitive and utilize instrumentation that is widely available. We previously took advantage of the reported reactivity of 8-oxodGuo to develop a method ...

We propose a novel semantic tagging task, sem-tagging, tailored for the purpose of multilingual semantic parsing, and present the first tagger using deep residual networks (ResNets). Our tagger uses both word and character representations, and includes a novel residual bypass architecture. We evaluate the tagset both intrinsically on the new task of semantic tagging, as well as on Part-of-Speec...

Purpose – The purpose of the paper is to provide an overview of the collaborative tagging phenomenon and explore some of the reasons for its emergence. The paper reviews the related literature and discusses some of the problems associated with, and the potential of, collaborative tagging approaches for knowledge organisation and general resource discovery. Design/methodology/approach – A defini...

Purpose – The purpose of the paper is to provide an overview of the collaborative tagging phenomenon and explore some of the reasons for its emergence. Design/methodology/approach – The paper reviews the related literature and discusses some of the problems associated with, and the potential of, collaborative tagging approaches for knowledge organisation and general resource discovery. A defini...

Biological discovery in the postgenomic era requires a systematic and high-throughput experimental approach. To this end, a versatile PCR-based tandem epitope tagging system is described, which inserts a tandem epitope coding sequence into any desired position of the Escherichia coli chromosome. Template plasmids were constructed that carry tandem copies of the epitope encoding sequence, Flp re...

The advent of super-resolution microscopy (nanoscopy) has set high standards for fluorescence tagging. Fluorescent proteins (FPs) are convenient tags in conventional imaging, but their use in nanoscopy has been questioned due to their relatively large size and propensity to form multimers. Here, we compared the nanoscale organization of proteins with or without FP tags by introducing the unnatu...

Previous stochastic localization-based super-resolution techniques are largely limited by the labeling density and the fidelity to the morphology of specimen. We report on an optical super-resolution imaging scheme implementing joint tagging using multiple fluorescent blinking dyes associated with super-resolution optical fluctuation imaging (JT-SOFI), achieving ultra-high labeling density supe...

Although a number of people have participated in tagging activity on many Web 2.0 sites and the sites allow users to get their tagging data through Mash-up APIs, there are many issues (e.g. tag semantics) associated with social tagging or folksonomies. Many studies have focused on semantics of a tag that offers a way of exact meaning of an individual tag or among the tags. Surprisingly, there h...

Data sparseness in tagging highly inflectional languages with large tagsets and scarce training resources is a problem that cannot be addressed using only common tagging techniques. Tiered tagging is a two-stage technique that uses for tagging a smaller ”hidden” tagset and, in the second phase, recovers the original tagset using a lexicon and a set of hand-written rules. The recovering is possi...

In this paper we analyse a social structure of an online community defined through tagging practices, and investigate whether useful knowledge about the evolution of social networks can be mined through tagging practices, and whether a more representative social structure has any influence on the tagging experience itself. The results from tagging behaviors also need to be represented semantica...