BACKGROUND Acute ruminal lactic acidosis (ARLA) is a major nutritional and metabolic disorder usually characterized by excessive or non-adapted intake of diets rich in nonstructural carbohydrates. Feed additives that regulate the ruminal environment have been used to prevent ARLA, such as ionophores and, more recently, yeast culture. Thus, we aimed to compare the efficacy of a yeast-based cultu...

The effect of monensin, a Na+-specific ionophore on the oxidative defense system in rat testis was studied. Monensin mixed in the animal diet was administered at the dose levels of 2.5, 5 and 10 mg/kg b.w. to Wistar rats for a period of 67 days. A marked inhibition in the activities of different oxidative defense enzymes such as catalase, glutathione peroxidase, glutathione-S-transferase, super...

Isometric force and 45Ca efflux from the sarcoplasmic reticulum were measured at 19 degrees C in frog skeletal muscle fibers skinned by microdissection. After Ca2+ loading, application of the ionophores monensin, an Na+(K+)/H+ exchanger, or gramicidin D, an H+ greater than K+ greater than Na+ channel-former, evoked rapid force development and stimulated release of approximately 30% of the accum...

The monovalent ionophore monensin inhibits the secretion of both procollagen and fibronectin from human fibroblasts in culture. The distribution of these proteins in control and inhibited (5 x 10(-7) M monensin) cells has been studied by immunofluorescence microscopy. In control cells, both antigens are present throughout the cytoplasm and in specific deposits in a region adjacent to the nucleu...

Monensin was fed at 0 and 33 ppm in a crossover trial designed to determine the effect of this compound on ruminal and postruminal utilization of a corn-based diet and bacterial protein synthesis in abomasally fistulated steers. Monensin decreased (P greater than .10) ruminal true digestion of organic matter (OMc, corrected for bacterial cell synthesis) and apparent ruminal digestion of starch ...

The collision-induced dissociation of lasalocid and monensin A bound to alkali metal cations has been investigated using electrospray MS/MS. The binding affinity for the metal cations, as measured with collision-induced dissociation, was found to depend on their ionic radius, decreasing with increasing radius. Monensin A was observed to have a greater binding affinity for alkali metal cations t...

UNLABELLED In our study, we characterized the effect of monensin, an ionophore that is known to raise the intracellular pH, on the hepatitis C virus (HCV) life cycle. We showed that monensin inhibits HCV entry in a pangenotypic and dose-dependent manner. Monensin induces an alkalization of intracellular organelles, leading to an inhibition of the fusion step between viral and cellular membranes...

  The efficacy of Clindamycin and Monensin were evaluated in the prevention of oocyst shedding of kittens with Toxoplasma gondii (Tehran strain). In this study, 28 healthy kittens aged 1.5 – 2 months old divided randomly into 4 groups of seven. In group 1, Kittens were fed with the infected brain tissues of mice, all of seven kittens (100 %), shed oocyst, nearly 1 week after infection, which la...

The ccr gene, encoding crotonyl coenzyme A (CoA) reductase (CCR), was cloned from Streptomyces cinnamonensis C730.1 and shown to encode a protein with 90% amino acid sequence identity to the CCRs of Streptomyces collinus and Streptomyces coelicolor. A ccr-disrupted mutant, S. cinnamonensis L1, was constructed by inserting the hyg resistance gene into a unique BglII site within the ccr coding re...

During the period from January to June, combined-sex broiler chickens were inoculated with coccidia via drinking water at 14 d of age. In a completely randomized design (eight replicate pens; 88 chicks per pen) using built-up litter, experimental diets contained monensin plus 0.20% dietary sodium bicarbonate (SBC), which provided 0.054% sodium and 0.144% bicarbonate. Treatment with SBC signific...