Genetic transformation of murine bone marrow stem cells to methotrexate resistance was achieved using a modified calcium phosphate-DNA coprecipitation procedure. Bone marrow cells were transformed by DNA derived from methotrexate-resistant mouse 3T6 cells. In vivo selection of drug-resistant bone marrow cells resulted from thrice weekly injections of methotrexate (MTX) for a period of 6-8 wk. F...

The objective of the current study was to investigate the ability of orthovanadate to induce aneuploidy in mouse sperm and micronuclei in mouse bone marrow cells at the same dose levels. The BrdU-incorporation assay was performed to test if the chemical treatment altered the duration of the meiotic divisions. It was found that orthovanadate (25mg/kg bw) treatment did not cause meiotic delay. To...

Background We intend to study the inhibitory effect of sulfur compound in Ramsar hot spring mineral on tumor-genesis ability of high natural background radiation. Objective The radioprotective effect of sulfur compounds was previously shown on radiation-induced chromosomal aberration, micronuclei in mouse bone marrow cells and human peripheral lymphocyte. Ramsar is known for having the highes...

Techniques for liquid culture of proliferating mononuclear phagocytes from bone marrow of mice and men are described. Mouse bone marrow must be cultured in the presence of colony-stimulating factor, whereas proliferation of human mononuclear phagocytes occurred in medium with 50% serum but without colony-stimulating factor. The number of mononuclear phagocytes that can be determined in mouse bo...

The development of colonies in nutrient rabbit, red cells exhibited enhancing agar in vitro from mouse hemopoietic ability, as did osmotically lysed erythrocells, in the presence of a maximal dose cyte preparations from rats. The use of of colony-stimulating activity (CSA), was red cells in the culture system, in addigreatly enhanced by the addition of lion to a maximum concentration of CSA, wa...

I N EARLIER REPORTS it was demonstrated that the injection of suspensions of normal bone marrow from the same species would prevent the death of animals exposed to total body irradiation. Data indicating protection are available on mice (I), rats (2), hamsters (3), and guinea pigs (I). The results obtained with bone marrow suspensions in irradiated rabbits and dogs were equivocal (49. 5>* Altho...

Mesenchymal stem cells (MSCs) differentiate into bone, fat, cartilage, tendon, and other organ progenitor cells. The rarity of MSCs in bone marrow necessitates fast and efficient isolation and/or in vitro expansion prior to clinical and biomedical applications. Previously, we reported that UV-exposed diphenylamino-s-triazine bridged p-phenylene vinylene (DTOPV-UV) with a hydrophilic and negativ...

The effects of mouse L-cell interferon (IFN) on growth of mouse bone marrow cells and their differentiation into macrophages and granulocytes were investigated in a liquid suspension culture system with two different types of colony-stimulating factor (CSF). Within 7 days, most bone marrow cells differentiated into macrophages in the presence of macrophage colony-stimulating factor (M-CSF) deri...

The production of erythrocytes bearing an "immature" antigen (Im+ cells) and a "fetal" antigen (Ft+ cells) has been studied in irradiated adult mice grafted either with fetal liver or adult bone marrow cells. The Im+ cells reach a peak 8-11 days after grafting. Ft+ cells are detected only after graft of fetal liver cells; the younger the liver, the greater the number. Since Ft+ cells are rapidl...

eighteen patients, twelve men and six women, with aplastic anemia underwent allogenic bone marrow transplantation (bmt) from hla-matched siblings during the period of 1990 to 1996. the conditioning regimen was cyclophosphamide with or without busulfan, depending on the cause of aplasia. antilymphocyte globulin (alg) and cyclosporine were used for rejection and acute gvhd prophylaxis, respective...