Primary bovine thyroid cell cultures and IB-RS-2 continuous cell line were used for foot-and-mouth disease virus (FMDV) isolation. In both cell culture systems, all tested samples gave positive results and the specificity of isolated virus was confirmed by the Ag-ELISA. Results of virus isolation test agreed with those obtained by RT-PCR and rRT-PCR, which enabled detection of the genetic mater...

Proposed changes to the International Animal Health Code chapter on foot and mouth disease (FMD) reflect both the introduction of new diagnostic tests to identify animals carrying live FMD virus, and pressure to make more acceptable the use of vaccination to help control outbreaks. It is important that those using the Code fully understand the implications of these changes. One of the proposed ...