We report West Nile virus (WNV) RNA in urine collected from a patient with encephalitis 8 days after symptom onset. Viral RNA was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Sequence and phylogenetic analysis confirmed the PCR product to have > or = 99% similarity to the WNV strain NY 2000-crow3356.

We evaluated the SD Bioline Influenza Ag A/B/A(H1N1) Pandemic test kit and compared it with real-time reverse transcriptase PCR (RT-PCR) for its ability to detect H1N1 2009. The sensitivity and specificity of the test kit for H1N1 2009 were 77% and 100%, respectively.

macrophage colony stimulating factor (m-csf) has previously been shown to affect the differentiation of cells of the mono-nuclear phagocytic line. more recent studies indicate that m-csf may have a role in pregnancy. in the present study, the expression of m-csf in the human placenta was demonstrated. placental mrna was isolated and used as template for synthesis of complementary dna (cdna). th...

We have developed a multiplex reverse transcription-polymerase chain reaction (RT-PCR) reaction, which enables us to detect 29 translocations/chromosomal aberrations in patients with acute lymphoid leukemia (ALL) and acute myeloid leukemia (AML). Through the construction and optimization of specific primers for each translocation, we have been able to reduce the set-up to 8 parallel multiplex P...

Abstract Background Real-time Reverse transcriptase—polymerase chain reaction (RT-PCR) is a regular technique for detecting SARS-CoV-2 infection. Human RNase P sequence using as internal control PCR assay usually. This article discusses the correlation between result and human gene result. Methods Nasopharyngeal swabs were collected from two patients within three consecutive days. We detected t...

A pandemia causada pelo agente etiológico SARS-CoV-2, teve início do ano de 2020 e gerou um grande desafio na comunidade científica todo o mundo. Seus sintomas podem variar manifestações leves à quadros severos pneumonia. Estudos estão buscando elucidar maneiras prevenção, diagnóstico tratamento da doença. Diante cenário pandêmico, agravamento no número casos óbitos pela doença, a busca por pre...

Following an approach used to specifically identify polioviruses and enterovirus 71, we have developed reverse transcriptase (RT) PCR primers containing mixed-base residues or deoxyinosine at positions of codon degeneracy. These primers permit specific RT-PCR amplification of echovirus 30 (E30) sequences by targeting sites that encode conserved amino acid motifs within the major capsid protein,...

A polymerase chain reaction (PCR) reactor was incorporated with an immunochromatographic ROSGENE strip for colorimetric detection of Influenza H1N1 virus. A reverse transcriptase-PCR (RT-PCR) cocktail, which contains Texas Redlabeled primers, biotin-dUTP, and Influenza H1N1 virus, was filled in the PCR chamber, and the target gene was amplified by RT-PCR. The resultant amplicons which were atta...