Pathways of electron transport have been studied in mitochondria isolated from hypocotyls of etiolated mung bean seedlings and skunk cabbage spadices that show cyanide-resistant respiratory activity. The residual flux through cytochrome c oxidase is shown to be small in comparison with the flux through an unidentified alternative oxidase that is known to have a high affinity for oxygen. This al...

5. GINSBURG, V., P. K. STUMPF, and W. Z. HASSID. 1956. The isolation of uridine diphosphate derivatives of D-glucose, D-galactose, D-xylose and L-arabinose from mung bean seedlings. Jour. Biol. Chem. 233: No. 2, 357-364. 6. HARRIS, G., J. W. DAVIs, and R. PARSONS. 1958. Nucleotide pool of brewer's yeast during growth and fermentation. Nature. 182: 1565-1567. 7. HENDERSON, F. J. and G. A. LEPAGE...

This study investigated the subcellular location of mung bean (Vigna radiata) 8S globulin in transient expression systems as well as in tobacco (Nicotiana tabacum) BY-2 cells and different tissues from a transgenic Arabidopsis (Arabidopsis thaliana) line stably expressing this storage globulin. When transiently expressed in protoplasts from both BY-2 cells and Arabidopsis suspension cultured ce...

Native granular starches (corn, cassava,mung bean, and sago) were hydrolyzed using amixture of alpha-amylase and glucoamylase at 35 ◦C for 24h. Hydrolyzed starches were analyzed for the degree of hydrolysis and for physicochemical and functional properties. Corn starch showed the highest degree of hydrolysis, as evidenced by the presence of distinct pores penetrating deep into the granules. Enz...

Vacuolar proton-translocating inorganic pyrophosphatase and H(+)-ATPase acidify the vacuoles and power the vacuolar secondary active transport systems in plants. Developmental changes in the transcription of the pyrophosphatase in growing hypocotyls of mung bean (Vigna radiata) were investigated. The cDNA clone for the mung bean enzyme contains an uninterrupted open reading frame of 2298 bp, co...

Arabinofuranosyltransferase activity was identified in Golgi membranes obtained from mung bean (Vigna radiata) hypocotyls. The enzyme transfers the arabinofuranosyl (Araf) residue from UDP-beta-L-arabinofuranose to exogenous (1, 5)-linked alpha-L-arabino-oligosaccharides labeled at their reducing ends with 2-aminobenzamide. The transferred residue was shown, using 1H-nuclear magnetic resonance ...

Terminal restriction fragment length polymorphism (T-RFLP) is a molecular technique used for comparative analysis of microbial community structure and dynamics. We evaluated three sampling methods for recovering bacterial community DNA associated with intestinal mucosa of mice (i.e. mechanical agitation with PBS, hand washing with PBS containing Tween 80, and direct DNA extraction from mucosal ...

Etiolated mung bean hypocotyl segments were incubated in 0.25 m mannitol solutions with indoleacetic acid. They were then deformed mechanically with a longitudinal tensile force at a constant strain rate. The magnitudes of the mechanical forces were comparable to those of the hydrostatic forces existing in normally growing tissues. Each segment was repeatedly deformed and returned to zero force...

Osmotic shock was found to be better than freezing and thawing, a French press, or sonic oscillation for the preparation of submitochondrial particles from mung bean (Phaseolus aureus) hypocotyl mitochondria. Particles prepared by osmotic shock rapidly oxidize reduced nicotinamide adenine dinucleotide and succinate, but they oxidize malate slowly. NADH oxidation was slightly stimulated by cytoc...