BACKGROUND Like other bacteria, Escherichia coli must carefully regulate the intracellular concentration of sodium ion (Na+). During the bacterial production of any organic acid, cations like Na+ invariably accumulate during a process which must maintain a near neutral pH. In this study, the E. coli nhaA gene encoding the Na+/H+ antiporter membrane protein and the nhaR gene encoding the NhaA re...

Na-driven fluid reabsorption prevents alveolar fluid accumulation. Hypoxia augments fluid filtration by hemodynamic effects and inhibits Na reabsorption in cultured cells. A combination of both effects in vivo causes alveolar flooding, edema, thickening of the diffusion barrier for oxygen, and hypoxemia, a life-threatening situation for mountaineers in remote high-altitude mountain ranges.

The vertebrate choroid plexus removes potentially toxic metabolites and xenobiotics from cerebrospinal fluid (CSF) to blood for subsequent excretion in urine and bile. We used confocal microscopy and quantitative image analysis to characterize the mechanisms driving transport of the large organic anion, fluorescein-methotrexate (FL-MTX), from bath (CSF-side) to blood vessels in intact lateral c...

Soil salinity and water deficit are major problems for development of agricultural production. In this study, effects of growth promoting microorganisms (inoculation with Piriformospora indica and two isolates of Streptomyces sp.) on the leaf yield and absorption rate of some elements in leaves and roots of stevia plant was investigated under salinity conditions for two consecutive years (2016-...

In the rat isolated optic nerve, nitric oxide (NO) activates soluble guanylyl cyclase (sGC), resulting in a selective accumulation of cGMP in the axons. The axons are also selectively vulnerable to NO toxicity. The experiments initially aimed to determine any causative link between these two effects. It was shown, using a NONOate donor, that NO-induced axonal damage occurred independently of cG...

The reversibility and regression of histological and biochemical findings in a mouse model of Gaucher disease (4L/PS-NA) was evaluated using a liver-enriched activator protein promoter control of a tetracycline-controlled transcriptional activation-responsive human acid beta-glucosidase (hGCase) transgenic system. 4L/PS-NA has the acid beta-glucosidase (GCase) V394L/V394L (4L) point mutation co...

In barley (Hordeum vulgare L.) leaves, differential ion accumulation commonly results in inorganic phosphate (Pi) being confined to the mesophyll and Ca(2+) to the epidermis, with preferential epidermal accumulation of Cl(-), Na(+), and some other ions. The pattern was confirmed in this study for major inorganic anions and cations by analysis of barley leaf protoplasts. The work focused on the ...

PURPOSE To determine the most appropriate time for laser irradiation to produce selective occlusion of new corneal vessels by photodynamic therapy (PDT) with a new photosensitizer, ATX-S10(Na). METHODS The time course of the plasma levels of ATX-S10(Na) and the degree of dye accumulation in the corneal neovascularization after intravenous administration was determined in rabbit eyes. Plasma c...

a hydroponic experiment was conducted to evaluate the effects of salt stress on safflower genotypes under greenhouse conditions. the experiment was arranged as a factorial one based upon a completely randomized design with three replications. five safflower genotypes (arak2811, nebraska10, sod25, ca116, and ca128), and four salinity treatments (0, 0.3, 0.5, and 0.7 percent of nacl) were employe...

The uphill transport of D-glucose in renal brush border membrane vesicles was correlated with the Na+ electrochemical gradient. Each component of the electrochemical potential, the membrane potential (outside positive) or the Na+ chemical gradient, when assayed independently, supported the concentrative uptake of the sugar. When the two components were combined, the rates of D-glucose uptake we...