objective: in this study, two conserved genes (m1 and np) of influenza virus were expressed in a bicistronic vector in order to develop a universal gene based vaccine. materials and methods: plasmids m1-pires2-egfp, pires2-np were constructed by cloning the pcr products of m1 and np genes which were amplified from the a/peurto rico/8/34 (h1n1) influenza virus strain into the plasmid expression...

detection of plasmodium vivax specific antibodies with serological tests could be a valuable tool for epidemiological researches. whereas p. vivax cannot be simply obtained in vitro, serological tests using total or semi-purified antigens are infrequently used. given this restriction, the present study investigated whether recombinant p. vivax merozoite surface protein 1 (pvmsp-1 42 kda) could ...

HIV-1 infection causes depletion and/or dysfunction of distinct CD4(+) T cell subsets and may affect these differently. Using the CD4C/HIV-1(Nef) transgenic (Tg) mice as a model, we report that HIV-1 Nef causes depletion of total CD4(+) T cells, but preserves and relatively enriches CD4(+) regulatory T cells (Treg). We found that Nef-mediated CD4(+) Treg enrichment is the direct result of Nef e...

The nef gene is important for the pathogenicity associated with simian immunodeficiency virus infection in rhesus monkeys and with human immunodeficiency virus type 1 (HIV-1) infection in humans. The mechanisms by which nef contributes to pathogenesis in vivo remain unclear. We investigated the contribution of nef to HIV-1 replication in human lymphoid tissue ex vivo by studying infection with ...

We compute Seshadri constants of a torus equivariant nef vector bundle on projective space satisfying certain conditions. As an application, we tangent bundles spaces. also consider Bott towers height 2 (i.e. Hirzebruch surfaces) and 3 respectively. Assuming some conditions the minimal slope restrictions these to invariant curves, give precise values constant at arbitrary point. several example...

BACKGROUND The Nef protein of HIV-1 is suspected to play a role in the depletion of uninfected CD4+ lymphocytes that leads to AIDS. By contrast its effect on CD8+ cells, whose functions are also deregulated during HIV-1 infection, is presently unclear. Here we describe a number of derangements induced in vitro by Nef in CD8+ cells from HIV-1-infected patients. DESIGN Peripheral lymphocytes fr...

The viral regulatory gene, nef, is unique to the human immunodeficiency viruses (HIV) and their related primate lentiviruses. Expression of the nef gene has been shown to be essential to the maintenance of high levels of virus replication and the development of pathogenesis in the animal model of simian immunodeficiency virus (SIV) infection. In contrast to this in vivo model, the use of standa...

The Nef protein of HIV-1 is essential for its pathogenicity and is known to down-regulate MHC expression on infected cell surfaces. We now show that Nef also redistributes the costimulatory molecules CD80 and CD86 away from the cell surface in the human monocytic U937 cell line as well as in mouse macrophages and dendritic cells. Furthermore, HIV-1-infected U937 cells and human blood-derived ma...

Eradication of the HIV-1 latent reservoir represents the current paradigm to developing a cure for AIDS. HIV-1 has evolved multiple mechanisms to evade CD8 T cell responses, including HIV-1 Nef-mediated downregulation of MHC-I from the surface of infected cells. Nef transcripts and protein are detectable in samples from aviremic donors, suggesting that Nef expression in latently HIV-1-infected ...

Tetherin, also known as BST2, CD317 or HM1.24, was recently identified as an interferon-inducible host-cell factor that interferes with the detachment of virus particles from infected cells. HIV-1 overcomes this restriction by expressing an accessory protein, Vpu, which counteracts tetherin. Since lentiviruses of the SIV(smm/mac)/HIV-2 lineage do not have a vpu gene, this activity has likely be...