Azospirillum is a rhizobacterial genus containing plant growth-promoting species associated with different crops worldwide. Azospirillum brasilense strains exhibit a growth-promoting effect by means of phytohormone production and possibly by N2 fixation. However, one of the most important factors for achieving an increase in crop yield by plant growth-promoting rhizobacteria is the survival of ...

We have developed a new primer design method based on the QuickChange site-directed mutagenesis protocol, which significantly improves the PCR amplification efficiency. This design method minimizes primer dimerization and ensures the priority of primer-template annealing over primer self-pairing during the PCR. Several different multiple mutations (up to 7 bases) were successfully performed wit...

PREMISE OF THE STUDY Microsatellite primers were developed for a widespread limestone endemic sedge, Carex eburnea, to facilitate investigation of the genetic diversity and phylogeography of this taxon and its closest relative, C. mckittrickensis. METHODS AND RESULTS Forty-eight primer pairs were designed from Illumina sequence data and screened for suitability. Fourteen of these primer pairs...

the genus artemisia containing more than 500 taxa at the specific or subspecific level. thirty-four species of genus artemisia have been reported in iran, which from the viewpoint distribution, density and coverage is the most important genera of plant. since the still has not been achieved a general system for the classification and phylogeny, in this research tried with evaluation of chloropl...

MOTIVATION Microarray experiments using probes covering a whole transcriptome are expensive to initiate, and a major part of the costs derives from synthesizing gene-specific PCR primers or hybridization probes. The high costs may force researchers to limit their studies to a single organism, although comparing gene expression in different species would yield valuable information. RESULTS We ...

A computer program is presented that selects a small set of short primer pairs for PCR to sample all the sequences in a user-specified list of mRNAs. Such primer pairs could be used to increase the probability of sampling mRNAs of particular interest in differential display and to generate simplified hybridization probes for DNA chips or arrays. The program uses simulated PCR to find pairs of p...

The NEW GIFT Nile tilapia is a national certificated new strain produced through 14 years and 9 generations selection from the base strain of GIFT Nile tilapia. From NEW GIFT Nile tilapia two strain-specific RAPD bands namely S304 and S36 were identified from the amplified bands of 50 and 80 10bp-oligo-nucleotide random primers separately after gel extraction cloning and sequencing of the strai...