An accurate high performance liquid chromatography method for the separation and quantification of a solution mixture of nitrazepam, diazepam and medazepam and medazepam was developed. The modified simplex program has been utilized for the optimization of the chemical and chromatographic parameters using the chromatographic response function as the quality criterion and a photodiode array as a ...

Background. HNF-1a is a transcription factor that regulates glucose metabolism by expression in various tissues. Aim. To dock potential ligands of HNF-1a using docking software in silico. Methods. We performed in silico studies using HNF-1a protein 2GYP·pdb and the following softwares: ISIS/Draw 2.5SP4, ARGUSLAB 4.0.1, and HEX5.1. Observations. The docking distances (in angstrom units: 1 angstr...

Using capillary electrophoresis combined with tandem mass spectrometry, we have developed a method to simultaneously detect lorazepam, clonazepam, temazepam, oxazepam, diazepam, and nitrazepam in urine. The urine samples were submitted to a modified QuEChERS extraction procedure, followed by electrophoretic separation in 0.1 M formic acid electrolyte (pH 2.4) using a polyvinyl alcohol (PVA)-coa...

We describe a simple, specific, and sensitive reversed-phase HPLC method with automated solid-phase extraction (SPE) for analyzing alprazolam, clonazepam, and nitrazepam concentrations in human serum or plasma. We prepare samples and calibrators with an automated sample preparer, using 100-mg Bond-Elut C18 SPE columns. The HPLC method uses isocratic elution with acetonitrile:methanol:dipotassiu...

The goal of the present study was to separate a set of benzodiazepines, namely bromazepam, oxazepam, nitrazepam, chlordiazepoxide, flunitrazepam, lormetazepam and diazepam by analytical scale HPLC and CZE. The both methods for separation of these seven compounds from the 1,4-benzodiazepine group were optimized and compared. LODs and LOQs were determined under the optimized conditions in the bot...

Xenobiotic metabolism involves the biochemical modification of drugs and phytochemicals in living organisms, including humans and other animals. In the intestine, the gut microbiota catalyzes the conversion of hydrophilic drugs into absorbable, hydrophobic compounds through hydroxyzation and reduction. Drugs and phytochemicals are transformed into bioactive (sulfasalazine, lovastatin, and ginse...