The terminal sugar sialic acid (Sia) plays a pivotal role in cell-cell interaction and recognition. A prerequisite for the biosynthesis of sialoglycoconjugates is the activation of Sia to cytidine monophosphate-Sia (CMP-Sia), by CMP-Sia synthetases (CMP-Sia-syn). CMP-Sia-syn are conserved from bacteria to man, and have been found to reside in the nucleus of all vertebrate species analysed to da...

The formation of singularities of self-focusing solutions of the nonlinear Schrodinger equation (NLS) in critical dimension is characterized by a delicate balance between the focusing nonlinearity and di raction (Laplacian), and is thus very sensitive to small perturbations. In this paper we introduce a systematic perturbation theory for analyzing the e ect of additional small terms on self fo...

The nuclear import of proteins larger than Mr 40,000 depends on the presence of a nuclear localization signal (NLS) corresponding either to a short peptide sequence or to defined sugars. The sugar-dependent nuclear import was previously evidenced by using glycosylated proteins (neoglycoproteins) introduced into the cytosol of cells either by electroporation or on digitonin-permeabilization and ...

The nuclear import of proteins typically requires the presence of a nuclear localization sequence (NLS). Some proteins have more than one NLS, but the significance of having multiple NLSs is unclear. The enzyme 5-lipoxygenase (5-LO) has three NLSs that, unlike the tight cluster of basic residues of the classical SV40 large T antigen NLS, contain dispersed basic residues. When attached to green ...

Pseudorabies virus (PRV) DNA replication occurs in the nuclei of infected cells and requires the viral DNA polymerase. The PRV DNA polymerase comprises a catalytic subunit, UL30, and an accessory subunit, UL42, that confers processivity to the enzyme. Its nuclear localization is a prerequisite for its enzymatic function in the initiation of viral DNA replication. However, the mechanisms by whic...

The pseudorabies virus (PRV) early protein UL54 is a homologue of herpes simplex virus 1 (HSV-1) immediate-early protein ICP27, which is a multifunctional protein that is essential for HSV-1 infection. In this study, the subcellular localization and nuclear import signals of PRV UL54 were characterized. UL54 was shown to predominantly localize to the nucleolus in transfected cells. By construct...

We constructed an underwater discharge system to conduct a number of experiments. Considering the constant resistance plasma channel, we got analytic expression for current containing unknown parameters on basis Kirchhoff voltage law. Therefore, are required determine total circuit R with measured data in hand. Three met...

We consider the defocusing cubic nonlinear Schrödinger equation (NLS) on twodimensional torus. The admits a special family of elliptic invariant quasiperiodic tori called finite gap solutions. These are inherited from integrable 1D model (cubic NLS circle) by considering solutions that depend only one variable. study long-time stability such for 2D and show that, under certain assumptions over ...

We study theoretically, numerically and experimentally the nonlinear propagation of partially incoherent optical waves in single mode optical fibers. We revisit the traditional treatment of the wave turbulence theory to provide a statistical kinetic description of the integrable scalar NLS equation. In spite of the formal reversibility and of the integrability of the NLS equation, the weakly no...

A single phosphorylation event at T-antigen residue Thr124 regulates initiation of simian virus 40 DNA replication. To explore this regulatory process, a series of peptides were synthesized, centered on Thr124. These peptides contain a nuclear localization signal (NLS) and a recognition site for cyclin/Cdk kinases. When unphosphorylated, the "CDK/NLS" peptides inhibit T-antigen assembly and bin...