Background: Three types of cell lines have been established from mouse blastocysts: embryonic stem (ES) cells, trophoblast stem (TS) cells, and extra-embryonic endoderm (XEN) cells, which have the potential to differentiate into their respective cognate lineages. ES cells can differentiate in vitro not only into somatic cell lineages but into extra-embryonic lineages, including trophectoderm an...

Topical issues related to the technology of isolation and mechanisms development pluripotent stem cells their application in medicine are considered. The isolation, as well subsequent use cells, still remains an unsolved problem both from a scientific point view especially practical health care. There three ways produce cells. First, they can be obtained vitro cell culture inner layer early egg...

background: the aim of this study was optimization of the polyfect gene delivery method of pcdna3.1 expression vector transfected with the mouse pdx-1 gene in three different kinds of mesenchymal stem cells and hepa cells as well as comparison of transfection efficiency leading to expression of the mentioned gene in the cell types used. materials and methods: rat bone marrow-derived mesenchymal...

BioTechniques 349 Embryonic stem (ES) cells were established from the inner cell mass of blastocysts and have been shown to spontaneously differentiate into multiple cell types representative of all three embryonic germ layers, both in teratomas after grafting or in vitro under appropriate conditions (1,2). ES cells can be maintained in their pluripotent state if cultured in the presence of leu...

The maintenance of pluripotential embryonic stem (ES) cells is dependent on the cytokine LIF. This report documents the mRNA expression profiles of LIF and the two components of the LIF-receptor complex, LIF-R and gp130, during early mouse embryogenesis. These mRNAs were undetectable in 1- or 2-cell embryos, but all were present by the blastocyst stage. LIF transcripts were localised in the dif...

BACKGROUND Until recently, the temporal events that precede the generation of pluripotent embryonic stem cells (ESCs) and their equivalence with specific developmental stages in vivo was poorly understood. Our group has discovered the existence of a transient epiblast-like structure, coined the post-inner cell mass (ICM) intermediate or PICMI, that emerges before human ESC (hESCs) are establish...

Mouse embryonic stem cells (mESCs), derived from the inner cell mass of the blastocyst, are pluripotent stem cells having self-renewal capability and the potential of differentiating into every cell type under the appropriate culture conditions. An increasing number of reports have been published to uncover the molecular mechanisms that orchestrate pluripotency and cell fate specification using...

Mouse Embryonic Stem Cells (ESCs) are pluripotent mammalian cells derived from the Inner Cell Mass (ICM) of mouse blastocysts, which give rise to all three embryonic germ layers both in vivo and in vitro. Mouse ESCs have a distinct epigenetic landscape and a more decondensed chromatin compared to differentiated cells. Numerous studies have shown that distinct histone modifications in ESCs serve...

objective: mir-302-367 is a cluster of polycistronic micrornas that are exclusively expressed in embryonic stem (es) cells. the mir-302-367 promoter is functional during embryonic development but is turned off in later stages. motivated by the cancer stem cell hypothesis, we explored the potential expression of mir-302 in brain tumor cell lines. materials and methods: in the present experimenta...

Analyses of gene expression in single mouse embryonic stem cells (mESCs) cultured in serum and LIF revealed the presence of two distinct cell subpopulations with individual gene expression signatures. Comparisons with published data revealed that cells in the first subpopulation are phenotypically similar to cells isolated from the inner cell mass (ICM). In contrast, cells in the second subpopu...