OBJECTIVE
To develop an in vitro strategy to support the growth of early-stage follicles and produce mature oocytes competent for fertilization.
DESIGN
Whole ovaries from 8-day-old mice were cultured for 4 days, and then secondary follicles were isolated and cultured for 12 days in a three-dimensional alginate or fibrin-alginate (FA) hydrogel matrix.
SETTING
University-affiliated laboratory...
