Multiple extracardiac stimuli, such as workload and circulating nutrients (e.g., fatty acids), known to influence myocardial metabolism and contractile function exhibit marked circadian rhythms. The aim of the present study was to investigate whether the rat heart exhibits circadian rhythms in its responsiveness to changes in workload and/or fatty acid (oleate) availability. Thus, hearts were i...

Fetuses of eight pregnant ewes (114-117 d of gestation) were used to study whether fetal insulin concentration affects fetal protein accretion and, if so, whether such changes are caused by effects on protein synthesis or protein breakdown. Fetal leucine kinetics were measured by infusion of [1-14C]leucine during each of three protocols: (I) low vs. normal insulin concentration; (II) low vs. hi...

To determine the rates of methionine splanchnic uptake and utilization in critically ill pediatric patients we used two kinetic models: the plasma methionine enrichment and the "intracellular" homocysteine enrichment. Twenty four patients, eight infants, eight children, and eight adolescents, were studied. They received simultaneous, primed, constant, intravenous infusions of l-[(2)H(3)]methylm...

Metabolic reprogramming, in which altered utilization of glucose and glutamine supports rapid growth, is a hallmark of most cancers. Mutations in the oncogenes KRAS and BRAF drive metabolic reprogramming through enhanced glucose uptake, but the broader impact of these mutations on pathways of carbon metabolism is unknown. Global shotgun proteomic analysis of isogenic DLD-1 and RKO colon cancer ...

hysteria is one of the conversion disorders that can be presented similar to neurological and organic disorders. conversion symptoms are usually associated with emotional conflicts of the patient. belching is often reported in patients with gastroesophageal reflux and is a rare manifestation of hysteria. the authors would describe a young female patient with serial belching that, after several ...

GThreonine dehydratase catalyzes the nonoxidative conversion of /Y-substituted amino acids to cr-keto acids. In this manuscript the nature of the nucleophiles which are eliminated is established, new substrates are reported, and inhibition by j&substituted alanines investigated. It is now shown that threonine dehydratase will catalyze the q/3 elimination of -OH, -Cl, and -F as HzO, and HX from ...

D-Amino acid oxidase catalyzes the nonoxidative conversion of oc-amino-P-chlorobutyrate to or-ketobutyrate, but does not catalyze its oxidation to P-chloroketobutyrate. The elimination reaction requires the oxidized flavoprotein, i.e. it does not occur with the apoenzyme or when the enzymebound flavin is reduced. The occurrence of this elimination reaction supports the proposal that removal of ...

The permanent draft genome sequence of Actinotignum schaalii DSM 15541T is presented. The annotated genome includes 2,130,987 bp, with 1777 protein-coding and 58 rRNA-coding genes. Genome sequence analysis revealed absence of genes encoding for: components of the PTS systems, enzymes of the TCA cycle, glyoxylate shunt and gluconeogensis. Genomic data revealed that A. schaalii is able to oxidize...

Kinetic and product analyses of the reactions of dialkylglycine decarboxylase with several alternative substrates are presented. Rate constants for the reactions of amino and keto acids of several substrates decrease logarithmically with increasing side-chain size. Conversely, kcat for L-amino acid decarboxylation increases with side-chain size. These and other data confirm a proposed model for...

Ethanol causes pancreatic damage by an unknown mechanism. Previously, we demonstrated that a sustained rise of the cytosolic Ca(2+) concentration ([Ca(2+)](i)) causes pancreatic acinar cell injury. Here we have investigated the effects of ethanol and its metabolites on Ca(2+) signaling in pancreatic acinar cells. Most cells exposed to ethanol (up to 850 mM) showed little or no increase in [Ca(2...