Understanding the relationship between the topological dynamics of nuclear subdomains and their molecular function is a central issue in nucleus biology. Pre-nucleolar bodies (PNBs) are transient nuclear subdomains, which form at telophase and contain nucleolar proteins, snoRNPs and pre-ribosomal RNAs (pre-rRNAs). These structures gradually disappear in early G1 phase and are currently regarded...

The critical maturation time of central otolith neurons in processing spatial orientations was examined in Sprague-Dawley rats. With the use of immuno-hybridization histochemical methods, we observed c-fos expression in vestibular nuclear neurons responding to transverse movement on the horizontal plane as early as P7 and those to antero-posterior stimulation as early as P9. In the inferior oli...

Acrylamide (ACR) is a chemical compound with severe neurotoxicity, genotoxicity, carcinogenicity and reproductive toxicity. Recent studies showed that ACR impairs the function of reproductive organs, e.g., epididymis and testes. In vitro maturation of mouse oocyte is a sensitive assay to identify potential chemical hazard to female fertility. The aim of this study was to evaluate the adverse ef...

BACKGROUND In vitro maturation (IVM) of oocytes followed by fertilization in vitro (IVF) and embryo transfer offers an alternative to conventional IVF treatment that minimises drug administration and avoids ovarian hyperstimulation. However, the technique is less efficient than maturation in vivo. In the present study, a non-human primate model was used to address the hypothesis that the number...

This study was conducted to investigate stimulatory effect of epidermal growth factor (EGF) on nuclear maturation and the expression level of EGF-receptor (EGFR), GM-130 (a marker of Golgi apparatus), transport protein Sec61 subunit beta (Sec61β), and coatomer protein complex subunit gamma 2 (COPG2) in porcine oocytes. The cumulus-oocyte complexes were collected from follicle with 3-6 mm in dia...

The biogenesis of Sm-type small nuclear ribonucleoproteins (snRNPs) involves the export of newly transcribed small nuclear RNAs (snRNAs) to the cytoplasm, assembly with seven common proteins and modification at the 5' and 3' termini. Binding of snRNP-specific proteins and snRNA modification complete the maturation process. This is thought to occur after reimport of the core snRNPs into the nucl...

22 23 The gGlycolysis and the pentose phosphate pathway (PPP) were modulated in porcine 24 cumulus-oocyte complexes (COCs) during in vitro maturation (IVM) by the addition of 25 inhibitors and stimulators of key enzymes of the pathways, with the aim of to analizing 26 analyze their participation influence on the oxidative status, mitochondrial activity and 27 maturation of the oocyte. The influ...

Background: Melatonin (N-acetyl-5-methoxytryptamine) is a multifunctional molecule that mediates several circadian and seasonal processes in animal reproduction. Melatonin and its metabolites are antioxidants and free radical scavengers. Furthermore, melatonin has been shown to act as an indirect antioxidant, and it is able to increase the activity of glutathione synthesis, and many others dire...

The present study aims to determine the potential of Bali cattle ovaries as sources oocytes for in vitro embryo production based on different ovarian reproductive statuses. were grouped into 4 categories: with no corpus luteum and dominant follicles (CL-DF-), those (CL+DF-), without but (CL-DF+), follicle (CL+DF+). collected via slicing technique grades (a, b, c, d). oocyte’s maturation was per...

Herpesviral capsid assembly is initiated in the nucleoplasm of the infected cell. Size constraints require that newly formed viral nucleocapsids leave the nucleus by an evolutionarily conserved vescular transport mechanism called nuclear egress. Mature capsids released from the nucleoplasm are engaged in a membrane-mediated budding process, composed of primary envelopment at the inner nuclear m...