This study found that in Tris-HCl buffer, the resonance light scattering (RLS) intensity of the Eu(3+)-nicotinic acid system can be greatly enhanced by nucleic acids and the enhanced intensity is proportional to the concentration of nucleic acid in the range of 7 x 10(-8)-1 x 10(-5 ) g x mL(-1) for fsDNA, and its detection limit is 2 x 10(-8 ) g x mL(-1). Based on this, a new method for the det...