Recently, biological techniques become more and more popular, as they are applied to many kinds of applications, authentication protocols, biochemistry, and cryptography. One of the most interesting biology techniques is deoxyribo nucleic acid and using it in such domains. Hiding secret data in deoxyribo nucleic acid becomes an important and interesting research topic. Some researchers hide the...

The next-generation sequencing technologies are able to produce millions of short sequence reads in a high-throughput, cost-effective fashion. The emergence of these technologies has not only facilitated genome sequencing but also started to change the landscape of life sciences. Here, I survey their major applications ranging from whole-genome sequencing and resequencing, single nucleotide pol...

Accurate and sensitive detection of Magnaporthe oryzae infection in agricultural crops is utmost significance for effective management as well restricting the dispersion pathogen to new terrains. The research progress field molecular biology, computational biotechnology, nanotechnology have provided opportunities develop disease specific diagnostic procedures. At present, nucleic acid driven pr...

Nucleobase analogs 5-methylisocytosine ((Me)isoC) and isoguanine (isoG) form a non-natural base pair in duplex nucleic acids with base pairing specificity orthogonal to the natural nucleobase pairs. Sequencing reactions were conducted with oligodeoxyribonucleotides (ODNs) containing d(Me)isoC and disoG using modified pyrosequencing and dye terminator methods. Modified dye terminator sequencing ...

The accuracy of nucleic acid sequence data interpretation was determined by assessing and quantifying the discrepancies reported in the GenBank database. This permitted the calculation of an Error Rate (ER) for nucleic acid sequence determination. If one assumes that most entries (TB, Total Bases) were independently verified or those without reported discrepancies were correct, the ER is 0.368 ...

Insensitivity and technical complexity have impeded the implementation of high-throughput nucleic acid sequencing in differential diagnosis of viral infections in clinical laboratories. Here, we describe the development of a virome capture sequencing platform for vertebrate viruses (VirCapSeq-VERT) that increases the sensitivity of sequence-based virus detection and characterization. The system...