The WRKY gene family of transcription factors is involved in several diverse pathways and includes components of plant-specific, ancient regulatory networks. WRKY genes contain one or two highly conserved DNA binding domains interrupted by an intron. We used partial sequences of five independent WRKY loci to assess their potential for phylogeny reconstruction. Loci were originally isolated from...

tuberculosis is a serious global public health problem and its high prevalence is stron gly associated with the enhancement of drug resistance. in this study we demonstrate a multiplex allele-specific polymerase chain reaction (mas)-pcr assay to simultaneously detect mutations in the first and third bases of the embb gene codon 306 atg in ethambutol (emb) resistant isolates of mycobacterium tub...

root rot caused by fusarium verticillioides is one of the most important rice diseases in ilam. in order to determine genetic diversity, 56 samples were collected from rice paddies of different regions in ilam province. molecular test was carried out with a set of five pairs of ssr primers after purification and identification of isolates. the ssr primers amplified a total 26 alleles. the avera...

background: hepatitis c virus (hcv) is one of the main causative factors of liver disease which can lead to chronic hepatitis c infection in 80% of cases. hcv genotypes have a special worldwide geographic distribution. the goal of the present study was to detect hcv genotypes in patients with anti-hcv positive titers in golestan province, iran. materials and methods: in this cross-sectional stu...

Calpastatin (CAST) is a specific inhibiter of Calpains, playing a role in meat tenderization and myogenesis. In the present study the polymorphism of the CAST gene ofKhalkhali goat in Azerbaijan province in Iran was investigated by polymerase chain reaction and restriction fragment length polymorphism technique (PCR-RFLP). Genomic DNA was extracted from whole blood samples collected from 200 Kh...

Several biological phenomena depend on differential methylation of chromosomal strands. While understanding the role of these processes requires information on allele-specific methylation, the available methodologies are not quantitative or labor-intensive. We describe a novel, rapid method to quantitate allele-specific DNA methylation based on the combination of bisulfite PCR and Pyrosequencin...

the diacylglycerol acyltransferase 1 gene (dgat1) was identified as a strong candidate gene affecting mutton quality traits in sheep. single nucleotide polymorphism creates a single base mutation (c to t) in agct site of endonuclease alui. dgat1 is one of the candidate genes to improve carcass characteristics in feedlot animals. in order to study area t487c in exon 17 of the dgat1 polymorphism,...

The role of 3' exonuclease excision in DNA polymerization was evaluated in primer extensions using 3' allele-specific primers that had exonuclease-digestible and exonuclease-resistant 3' termini. With exonuclease-digestible unmodified 3' mismatched primers, the exo+ polymerase yielded template-dependent products. Using exonuclease-resistant 3' mismatched primers, no primer-extended product resu...