Mice lacking the oxalate transporter SLC26A6 develop hyperoxalemia, hyperoxaluria, and calcium-oxalate stones as a result of a defect in intestinal oxalate secretion, but what accounts for the absorptive oxalate flux remains unknown. We measured transepithelial absorption of [(14)C]oxalate simultaneously with the flux of [(3)H]mannitol, a marker of the paracellular pathway, across intestine fro...

H2O2-driven enzymes are of great interest for industrial biotransformations. Herein, we show the first time that oxalate oxidase (OXO) is an efficient in situ source H2O2 one these biocatalysts, which known as unspecific peroxygenase (UPO). OXO reasonably robust, produces only CO2 a by-product and uses cheap sacrificial electron donor. UPO has significant potential catalyst selective C−H oxyfun...

Background American chestnut (Castanea dentata) once dominated the forests of the eastern United States until the introduction of a Chinese fungal pathogen Cryphonectria parasitica(causal agent of the chestnut blight) decimated the species. This tree was important not only ecologically, but also played a significant role economically. Restoration programs are implementing a variety of technique...

Germin with oxalate oxidase and superoxide dismutase activity is a homohexamer of six manganese-containing interlocked beta-jellyroll monomers with extreme resistance to heat and proteolytic degradation [Woo, E.-J., Dunwell, J. M., Goodenough, P. W., Marvier, A. C. & Pickersill, R. W. (2000) Nat. Struct. Biol. 7, 1036-1038]. This structure is conserved in germin-like proteins (GLPs) with other ...

This paper describes characteristics of the transport of oxalate across the human erythrocyte membrane. Treatment of cells with low concentrations of H2DIDS (4,4'-diisothiocyanatostilbene-2,2'-disulfonate) inhibits Cl(-)-Cl- and oxalate-oxalate exchange to the same extent, suggesting that band 3 is the major transport pathway for oxalate. The kinetics of oxalate and Cl- self-exchange fluxes ind...

BACKGROUND The role of calcium oxalate crystals and deposits in UTI pathogenesis has not been established. The objectives of this study were to identify bacteria present in pediatric urolithiasis and, using in vitro and in vivo models, to determine the relevance of calcium oxalate deposits during experimental pyelonephritis. METHODS Pediatric kidney stones and urine were collected and both cu...

Oxalate is a normal constituent of human urine. Since oxalate is found in many foods it has frequently been assumed that urinary oxalate is largely, if not entirely, of dietary origin. However, animal studies have shown that oxalate may arise endogenously, and glycine, glyoxylic acid, glycolic acid (3-5) and ascorbic acid (6, 7) have been shown to be precursors of urinary or tissue oxalate unde...

In this study, the specific contribution of polyamine oxidase (PAO), a hydrogen peroxide (H2O2)-producing enzyme, to the oxidative burst induced in maize mesocotyl by the phosphatase inhibitor cantharidin was examined. For this purpose, a pharmacological approach was applied using, either in vitro or in vivo, two strong inhibitors of maize PAO (MPAO), N-prenylagmatine (G3) and its structural an...