Background and Objective: Formalin-fixed paraffin-embedded tissues are a valuable source of DNA for molecular studies. We designed and optimized an efficient procedure for DNA extraction from formalin-fixed paraffin embedded tissues. Materials and Methods: Seventy three blocks of cervical paraffin-embedded tissues were investigated. DNA was extracted using 45 minutes boiling in alkaline sol...

introduction : the aim of this study was to examine and compare the apical sealing ability of ah26, ah plus and ah plus jet using a fluid filtration model. materials and methods : 70 single-rooted teeth were dissected from the cement-enamel junction. canals were prepared with protaper rotary system and manual k files and irrigated with 5.25% naocl and 17% edta. ten teeth were assigned as contro...

abstract the aim of this experiment was the study of freezing tolerance of bellis perennis under controlled conditions and were arranged in a completely randomized design with three replications. plants after sowing and grow in the bed, at the middle of autumn (after hardening in natural conditions), in the 7-8 leaf stage put on the thermogradiant freezer with the 12 freezing temperatures (0,-2...

objective: the aim of this study is to measure the effect of different condensation pressure on the microleakage of mineral trioxide aggregate (mta).  methods: this study was an invitro study. a total of 55 single rooted straight-canal human extracted sound teeth free of cracks or calcification were selected. teeth were sectioned from cej region at 3 mm above the root apex. remaining specimens ...