Continuing threats of devastating outbreaks in poultry and of human infections caused by highly pathogenic avian influenza virus (HPAIV) H5N1 emphasize the need for the further development of rapid and reliable methods of virus detection and characterization. Here we report on the design and comprehensive validation of a low-density microarray as a diagnostic tool for the detection and typing o...

Real-time reverse transcription polymerase chain reaction (RT-PCR) methods that monitor product accumulation were adapted for the validation of differentially expressed genes. We describe a real-time quantitative PCR assay that uses SYBR Green I dye-based detection and product melting curve analysis to validate differentially expressed genes identified by gene expression profiling technologies....

We present a method for performing highly parallel PCR reactions in a picowell array (PWA) simultaneously immobilizing generated PCR products in a covalent and spatially-resolved manner onto a microscope slide via solid-phase PCR (SP-PCR). This so called PWA-SP-PCR was performed in picowell arrays featuring 100,000 wells cm(-2) of 19 pL reaction volumes with a surface-to-volume ratio of 0.2 μm(...

We generalize the notion of Jacobson graphs into $n$-array columns called $n$-array Jacobson graphs and determine their connectivities and diameters. Also, we will study forbidden structures of these graphs and determine when an $n$-array Jacobson graph is planar, outer planar, projective, perfect or domination perfect.

Given four complex matrices $A$‎, ‎$B$‎, ‎$C$ and $D$ where $Ainmathbb{C}^{ntimes n}$‎ ‎and $Dinmathbb{C}^{mtimes m}$ and let the matrix $left(begin{array}{cc}‎ A & B ‎ C & D‎ end{array} right)$ be a normal matrix and‎ assume that $lambda$ is a given complex number‎ ‎that is not eigenvalue of matrix $A$‎. ‎We present a method to calculate the distance norm (with respect to 2-norm) from $D$‎ to ...

The interpretation of oligonucleotide array experiments depends on the quality of the target cRNA used. cRNA target quality is assessed by quantitative analysis of the representation of 5' and 3' sequences of control genes using commercially available Test arrays. The Test array provides an economically priced means of determining the quality of labeled target prior to analysis on whole genome ...

in this work, some of the lattice plasmon quantum features are examined. Initially, the interaction of the far-field photonic mode and the nanoparticle plasmon mode is investigated. We probe the optical properties of the array plasmon that are dramatically affected by the array geometry. It is notable to mention that the original goal of this work is to examine the quantum feature of the array ...

Endometriosis is a painful chronic female disease defined by the presence of endometrial tissue implants in ectopic (Ec) locations. The pathogenesis is much debated, and type-I interferons (IFNs) could be involved. The expression of genes of the type-I IFN response were profiled by a specific PCR array of RNA obtained from Ec and eutopic (Eu) endometrium collected from nine endometriosis patien...

Abstract— This work investigates the improvement of the active reflection coefficient of waveguide-fed phased-array antenna using liquid crystal layers. The anisotropy properties of liquid crystal layer can be employed to eliminate blind scan angle and improve the wide angle impedance matching of the waveguide-fed phased array antennas. The authors have expressed the modal analysis of the waveg...