Background Both real-time-polymerase chain reaction (PCR) and hybrid capture 2 (HC2) assay can detect and quantify hepatitis B virus (HBV) DNA. However, real-time-PCR can detect a wide range of HBV DNA, while HC2 assay could not detect lower levels of viremia. The present study was designed to detect and quantify HBV DNA by real-time-PCR and HC2 assay and compare the quantitative data of these ...

INTRODUCTION Herein, we report a one-tube, semi-nested-polymerase chain reaction (OTsn-PCR) assay for the detection of Paracoccidioides brasiliensis. METHODS We developed the OTsn-PCR assay for the detection of P. brasiliensis in clinical specimens and compared it with other PCR methods. RESULTS The OTsn-PCR assay was positive for all clinical samples, and the detection limit was better or ...

Objectives: Detection of Yersinia enterocolitica in clinical samples is still not sensitive and fast enough. Polymerase chain reaction (PCR) offers the advantages of sensitivity, specificity, and rapidity. A two-step PCR assay to detect pathogenic Y: enterocolitica in infected tissue has been developed. Method: In the first step of the PCR assay, a general primer PCR amplification of the small ...

Foot-and-mouth disease virus (FMDV), Bluetongue virus (BTV), Vesicular stomatitis Virus (VSV), Bovine viral diarrheal (BVDV), Bovine rotavirus (BRV), and Bovine herpesvirus 1 (IBRV) are common cattle infectious viruses that cause a great economic loss every year in many parts of the world. A rapid and high-throughput GenomeLab Gene Expression Profiler (GeXP) analyzer-based multiplex PCR assay w...

The performance of the Illumigene group A Streptococcus assay was evaluated by comparing it to culture using 437 consecutive throat swabs. The Illumigene assay was also directly compared to PCR with 161 samples. This Illumigene assay is rapid and easy to perform. The assay also has high sensitivity (100%) compared to culture or PCR and high specificity (99.2%) compared to PCR. A total of 8.8% o...

Respiratory tract infections are one of the most important among military personals, worldwide. The present study aimed to survey prevalence bacterial and viral main etiological agents causing respiratory personnel in Iran. A cross-sectional was performed from November 2020 March 2021. Nasopharyngeal swabs were taken patients, with infection symptoms. Detection coronavirus disease 2019 (COVID-1...

The hologic panther fusion (PF) platform provides fully automated CE marked diagnostics for respiratory viruses, including the recently discovered severe acute syndrome-coronavirus 2 (SARS-CoV-2) by a transcription mediated amplification (TMA) assay, but not endemic human coronaviruses (hCoV). Therefore, laboratory developed test (LDT) comprising multiplexed reverse polymerase chain reaction (R...

Female genital tuberculosis (FGTB) has a crucial role in many gynaecological complaint and delay diagnosis can cause irreversible damages. The new diagnostic technique GeneXpert system is accurate rapid test make paradigm shift FGTB treatment. aim of the study to estimate efficacy different tests i.e. AFB BACTEC culture, molecular assay (TB-PCR) histopathology comparison detect FGTB. prospectiv...

Cytomegalovirus (CMV) infection is an important cause of morbidity and mortality after allogeneic hematopoietic stem cell transplantation. Therefore, preemptive ganciclovir therapy based on early detection of CMV reactivation is widely used to prevent CMV disease. Real-time polymerase chain reaction (PCR) has been widely used for monitoring CMV reactivation as well as the antigenemia assay that...

Polymerase chain reaction (PCR) amplification of DNA was used to detect the etiologic agent of murine typhus, Rickettsia typhi, in experimentally infected adult fleas. A primer pair derived from the 17-kilodalton antigen sequence of typhus and spotted fever group rickettsiae was used to amplify a 434-base-pair (bp) fragment of the genome of the murine typhus rickettsiae. The amplified 17-kiloda...