PCR fingerprinting was used to type 177 yeast isolates obtained from two medical institutions. Candida albicans was the predominant species found, followed by C. tropicalis, C. glabrata, C. parapsilosis, C. guilliermondii, and C. krusei, which accounted for over 20% of the strains isolated. This survey represents the first study of molecular epidemiology of candidiasis in Portugal.

Four patients in an oncology ward developed Bacteroides fragilis bacteremia over a 12-day period. Cross-infection between two of them, due to an imipenem-resistant strain, was demonstrated by epidemiological investigation and genotypic typing methods (arbitrarily primed PCR fingerprinting and nucleotide sequencing of the cfiA genes and upstream IS1186/IS1168 elements).

Fifty-eight vancomycin-resistant enterococcal isolates were obtained from two patients over 9 weeks. Numerous pulsed-field gel electrophoresis fingerprinting types were isolated from each patient. By PCR, all isolates were vanA+. However, many isolates from patient B were found to lack vanA by hybridization. Our results demonstrate the importance of examining multiple isolates, especially from ...

A ligation-mediated PCR (LMPCR) method for the amplification of sequences flanking the IS6110 of the Mycobacterium tuberculosis complex has been developed. The method uses one primer specific for IS6110 and a second specific for a linker ligated to SalI-restricted genomic DNA. LMPCR is a rapid screening method, valuable for the fingerprinting of M. tuberculosis complex strains.

conclusions: the combination of plasmid profiling and php techniques revealed the presence of diverse population of vre in sewage treatment plant in tehran. furthermore, the results showed that the php technique is a reliable method in determining the vre clonal diversity. results: all isolates were found to be resistant to erythromycin, ampicillin and ciprofloxacin. the pcr analyses showed tha...

The purpose of this article is to review the status of DNA assays used for the detection, identification, and individualization of Bacillus anthracis, Yersinia pestis, Francisella tularensis, Burkholderia mallei, and Brucella abortus. These select agent microorganisms are historically significant as they have either been used or experimented with as a bioweapon or as a terrorist agent and are t...

A total of 49 type and neotype isolates and 32 clinical isolates of the anamorph genus Candida and related teleomorph genera were obtained from different culture collections and clinical laboratories. Isolates were subjected to two phenotypic methods of identification, Vitek yeast biochemical card (YBC) and API ID 32C, both based on carbohydrate assimilation, and one genotypic method, PCR finge...

today fingerprinting techniques are increasingly adopted as an alternative and more direct and reliable means of assembling sediment source information. one of the principal assumptions of sediment fingerprinting is that potential catchment sediment sources can be distinguished on the basis of their physical, geochemical and biological properties or fingerprint properties. however, while the so...

Pseudomonas strains originating from symptomatic (bacterial spot) leaf tissues of sweet cherry (Topola, Sumadija) and plum (Krusedol Selo, Srem) were isolated during 2016 2020, respectively. Based on the findings yielded by classical microbiological methods, LOPAT (+---+), GATTa (--++) pathogenicity tests performed detached fruitlets (sweet sour cherry) pods (bean pods), all confirmed to belong...