Porcine DNA authentication in commercial products is critical due to improper or non-certified Halal logo on its packaging. This study detected the presence of porcine particularly processed meat sold Sarawak. A total 75 samples were collected, consisting poultry, beef, and seafood products. was isolated amplified a polymerase chain reaction that targeted cytochrome B (cytb) mitochondrial D-loo...

Serpula himantioides is a wood-decaying fungal pathogen which widespread and causes potentially serious butt rot in living trees of numerous coniferous plantation species. This study aimed to quantify S. the wood Sawara cypress (Chamaecyparis pisifera) with using real-time polymerase chain reaction (real-time PCR). Species–specific primers were designed for internal transcribed spacer 2 (ITS2) ...

The commercialization of GE crops requires a rigorous safety assessment, which includes precise DNA level characterization inserted T-DNA. In the past, several strategies have been developed for identifying T-DNA insertion sites including, Southern blot and different PCR-based methods. However, these methods are often challenging to scale up screening dozens transgenic events with complex genom...

Three sets of polymerase chain reaction (PCR) primers were designed for heminested PCR amplification of the target DNA fragments in the human genome which include the site of BRCA1 c.68_69del, BRCA1 c.5266dup and BRCA2 c.5946del respectively, to prepare the templates for direct Sanger sequencing screen of these three founder mutations. With a robust PCR mixture, crude proteinase K digestate of ...

Targeted DNA enrichment coupled with next generation sequencing has been increasingly used for interrogation of select sub-genomic regions at high depth of coverage in a cost effective manner. Specificity measured by on-target efficiency is a key performance metric for target enrichment. Non-specific capture leads to off-target reads, resulting in waste of sequencing throughput on irrelevant re...

In this study the genetic diversity of wild Caspian trout (Salmo trutta caspius) in the Sardabroud and Astara Rivers was evaluated using D- Loop region sequencing. For this purpose, 35 specimens of adult Caspian brown trout were collected from these rivers in the Mazandarn and Gilan Provinces in fall and winter 2011. Approximately 3-5 g of soft and fresh fin tissue was isolated and fixed in eth...

Genetic diversity as an important marker of the ecological status of aquatic ecosystems is considered a unique and powerful tool to evaluate biological communities. In order to evaluate the genetic diversity among golden mullet species (Liza aurata) in the southeast and southwest coasts of the Caspian Sea by D-Loop gene sequencing, a total of 23 fin specimens of golden mullet were collected fro...

Gene polymorphisms and mutations have been correlated to different diseases and clinical syndromes. Therefore, screening for gene mutations is an appropriate approach to researching disease. The location of mutation hot spots is not known in new or rarely analyzed genes. To locate such hot spots, the screening of a complete gene sequence in a representative number of individuals is required. Se...

An efficient approach for structural studies on bacterial chromosomes is presented. It is based on high-resolution PCR map construction by using a multiplex long accurate PCR (MLA PCR) protocol and a YAC clone carrying the region to be mapped as indicator. The high-resolution PCR map of the bacillus subtilis rrnB-dnaB region is presented as an example. Data are also presented on the use of DNA ...

We describe a method to ligate a PCR-amplified DNA fragment with T-protruding cassettes, which have multiple sites for endonuclease, promoter sequences of T3 and T7, and annealing sites for the universal M13 forward and reverse primers. This method, which we named T-cassette ligation, substantially facilitated direct sequencing and subcloning of PCR products. Two T-cassettes with a protruding T...