نتایج جستجو برای: penicillin acylase

تعداد نتایج: 19908  

Journal: :Applied and environmental microbiology 2007
Tamara Montes Valeria Grazú Fernando López-Gallego Juan A Hermoso Jose L García Isabel Manso Beatriz Galán Ramón González Roberto Fernández-Lafuente José M Guisán

A new mutant of the industrial enzyme penicillin G acylase (PGA) from Escherichia coli has been designed to improve its reversible immobilization on anionic exchangers (DEAE- or polyethyleneimine [PEI]-coated agarose) by assembling eight new glutamic residues distributed homogeneously through the enzyme surface via site-directed mutagenesis. The mutant PGA is produced and processed in vivo as i...

2007
L. M. Pinotti R. G. Silva T. C. Zangirolami L. C. Giordano

This work reports the influence of different culture preservation methods on the production of penicillin G acylase (PGA) by Bacillus megaterium ATCC 14945. The initial stock culture, presenting PGA activity of 97 IU l, was preserved for two years using different procedures: monthly subculturing and storage in refrigerator (S), freeze-drying using skim milk 10%, plus inositol 5% as cryoprotecto...

Journal: :Acta Crystallographica Section F Structural Biology and Crystallization Communications 2011

Journal: :Acta Crystallographica Section A Foundations of Crystallography 1996

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