BACKGROUND It has been demonstrated that growth hormone (GH) is synthesized and secreted by human peripheral mononuclear cells (PBMC), and the expression of GH mRNA can be found throughout the human immune system. METHODS We studied a population of female caregivers of patients with Alzheimer's dementia (AD) who suffered from the stress of caring for these patients. We utilized quantitative R...

Methods The HIV inhibitory activity of aqueous and methanolic extracts of S. swartzii was determined on laboratory adapted strains of HIV-1 clades C and A by indirectly measuring reduction in HIV-1 gag p24 antigen levels and inhibition of HIV-1 RT activity in supernatants of virus infected donor peripheral blood mononuclear cell (PBMC) cultures grown in varying concentrations of the extracts. P...

We studied the effect of sulfurous water thermal therapy on the phenotype and the proliferative response of peripheral lymphoid cells from ten subjects affected by chronic upper respiratory disease and from six suffering from articular and periarticular disorders. Sulfurous water (S-H2O) therapy did not modify the phenotype and function of peripheral blood mononuclear cells (PBMC) nor did it mo...

We synthesized and cloned cDNA from human peripheral blood mononuclear cell (PBMC) transcripts that were hybrid selected by pCRP5, a liver C-reactive protein (CRP)-specific cDNA (Woo, P., J.R . Korenberg, and A.S. Whitehead . 1985 . J. Biol. Chem . 260:13384) . Three hybridselected cDNA clones, HScDNA1, HScDNA3, and HScDNA8, were isolated and characterized . Nucleotide sequence analysis of the ...

We previously demonstrated that stimulation of bovine peripheral blood mononuclear cells (PBMCs) with staphylococcal enterotoxin C (SEC), led to an inversion of the CD4(+):CD8(+)T cell ratio and generation of an atypical CD8(+)T cell subpopulation expressing CD26. In the present study, we examined T cell apoptosis and proliferation profiles of PBMC subpopulations in cultures stimulated with SEC...

To test the Efficacy of outpatient intracervical injection of peripheral blood mononuclear cells in the treatment of habitual abortion cervical insufficiency. Pilot study Heliopolis research center and Heliopolis Hospital, Cairo, Egypt Preparation of peripheral blood mononuclear cells, transvaginal ultrasound, IL-8, collagenases in the cervical mucus, Aquaporins in the cervical smears before PB...

Plasma human immunodeficiency virus (HIV) type 1 RNA load is the reference marker for response to antiretroviral therapy. To compare peripheral blood mononuclear cell (PBMC)-associated and plasma HIV-1 RNA response to treatment, HIV-1 RNA was quantified by reverse transcription-competitive polymerase chain reaction in 20 patients at 0, 12, and 24 weeks following addition of saquinavir to their ...

The influence of graft composition on clinical outcomes after reduced-intensity conditioning is not well-characterized. In this report we prospectively enumerated CD34 , CD3 , CD4 , and CD8 cell doses in granulocyte colony-stimulating factor– mobilized peripheral blood mononuclear cell (G-PBMC) allografts in 63 patients who received transplants following nonmyeloablative conditioning with total...

THIS flow cytometry antibody panel was developed and optimized for the characterization of CD41 and CD81 T-cell memory and functional responses in adult and infant cryopreserved peripheral blood mononuclear cells (PBMC) stimulated with peptide pools to various antigens of interest (Table 1). The panel has been used to evaluate Mycobacterium tuberculosis (TB) antigen-specific responses in clinic...

During gene therapy trials, immune responses against adeno-associated virus (AAV) vectors are monitored by antibody assays that detect the humoral and T-cell mediated cellular to AAV vectors. T cell commonly utilize collection of patients’ peripheral blood mononuclear cells (PBMCs) stimulation with AAV-derived overlapping peptides. We recently described spontaneous deamidation coincides epitope...