Development of sensitive enzymatic methods for hydrogen peroxide is key for the quantification of several bioconstituents such as glucose, triglycerides, creatinine, and uric acid and so on. Hydrogen peroxide released by the oxidase enzymes are quantified by peroxidase enzyme involving spectrophotometry, fluorimetry, chemiluminisence, potentiometric sensing, amperometric, coulometric and such o...

BACKGROUND This investigation describes the relationship between glutathione peroxidase activities, an antioxidant enzyme, and the oxidative status in diabetic rat blood. METHODS Malondialdehyde level and glutathione peroxidase activity were measured by spectrophotometric techniques. RESULTS AND CONCLUSIONS Malondialdehyde content in the diabetic rats group was increased compared to that in...

BACKGROUND The alterations of the glutathione peroxidase enzyme complex system occur in physiological conditions such as aging and oxidative stress consequent to strenuous exercise. METHODS Authors optimize the spectrophotometric method to measure glutathione peroxidase activity in rat red blood cell membranes. RESULTS The optimization, when applied to age paired rats, both nulligravid and ...

The white rot fungus Pycnoporus cinnabarinus was characterized with respect to its set of extracellular phenoloxidases. Laccase was produced as the predominant extracellular phenoloxidase in conjunction with low amounts of an unusual peroxidase. Neither lignin peroxidase nor manganese peroxidase was detected. Laccase was produced constitutively during primary metabolism. Addition of the most ef...

Peroxidases are the ubiquitous enzyme and reported to be present in all living genera. They catalyses reduction of peroxide and generate reactive oxygen species. In the present study we demonstrated that insect infestation induces peroxidase activity in sap and total soluble protein (TSP) of plant leaves. Three important crop plants viz. tomato, cowpea and cotton were used for this study. After...

Changes in peroxidase activity as well as isozyme peroxidase pattern were analyzed in five cell wall (CW) fractions of barley roots exposed to 1mM CdCl2 for 48 and 72 h. Whereas strong inhibition of peroxidase activity was detected in fraction CW I and weak inhibition in fractions CW II, III and IV, strong activation of peroxidase was observed in fraction V after Cd application. Despite the inh...

Four selenocysteine-containing proteins (gastrointestinal glutathione peroxidase, plasma glutathione peroxidase, selenoprotein P, and thioredoxin reductase-alpha) are expressed in the colonic mucosa. Because of their antioxidant functions, a protective role in colon carcinogenesis is discussed. The aim of this study was to elucidate an involvement of gastrointestinal selenoproteins during the a...

Application of nanotechnology is crucial for a sustainable increase in food production to cope with the increasing demand burgeoning population. Wheat has significantly security Pakistan help nanotechnology. In biological systems, utilization nanoparticles been increased due their growth-promoting effects on germination, photosynthetic attributes, nutrient use efficiency and metabolic activitie...

The Plant peroxidases are remarkable enzymes due to their widespread use in industry. These enzymes, which capable of catalyzing the oxidation various organic and inorganic substrates, have been used clinical diagnosis, detoxification reactions synthesis. In this study, vitro production purification peroxidase enzyme from cauliflower plant was proposed. Firstly, sterile seedlings were obtained ...