Experiments were carried out in vivo to investigate the pathways of ammonia incorporation into rumen bacteria, bacterial fractions and free amino acids within the bacteria. Steers were alternately given two isoenergetic, isonitrogenous diets containing the nitrogen mainly as either urea or decorticated groundnut meal (DCGM). At the end of each period on a given diet, a solution of 15NH4Cl was i...

Two trials were conducted to study ruminal lactic acid, histamine, tyramine and tryptamine concentrations following glucose engorgement in vitro and in vivo. Also, electrolyte changes in rumen fluid, blood plasma and urine during lactic acidosis were examined. Experiment 1 involved the addition of glucose to rumen fluid in vitro. After 30 hr of incubation, decreases in pH and increases in lacti...

Dry matter and cellulose digestibility were not affected by the urea infusions. The apparent nitrogen digestibility, derived from the difference between feed and faecal N, and ignoring infused N, was slightly depressed by urea infusions of from 9 to 34 g N/day, but at the highest level of infusion (43.7 g N/day) it was equal to or greater than the control value. Rumen pH and VFA concentrations ...

This study was conducted to evaluate the possibility of alfalfa replacement with fenugreek hay for ruminants, using in vitro gas production parameters and some of rumen fermentation characteristics. For this propose, five different total mixed rations were formulated to meet nutrient requirements of 35 kg growing lambs with gradually replacement of alfalfa in control diet (T1) with different le...

Short term in vitro incubations were used to evaluate the effect of fumaric acid bentonite coupled addition on rumen fermentation effeciency. Ruminal contents from five steers were used for preparation of inoculums of mixed rumen microorganisms that were used in generatation of three treatment systems, negative control, fumaric acid treated, and fumaric acid – bentonite coupled treated. The fer...

Urease (urea amidohydrolase, EC 3.5.1.5) was extracted from the mixed rumen bacterial fraction of bovine rumen contents and purified 60-fold by (NH4)2SO4 precipitation, calcium phosphate-gel adsorption and chromatography on hydroxyapatite. The purified enzyme had maximum activity at pH 8.0. The molecular weight was estimated to be 120000-130000. The Km for urea was 8.3 X 10(-4) M+/-1.7 X 10(-4)...

Therapeutic efficacy of certain poly herbal formulations and probiotics along with heterologous cud transplantation was evaluated in simple indigestion in buffaloes. A total of 18 buffaloes suffering from simple indigestion were treated with Suruchi bolus (10 g twice daily) or Gastricare powder (15g twice daily) or Yeasacc bolus with heterologous cud from healthy sheep. Clinical examination and...

Story in Brief The phosphorus concentration in ruminal fluid was determined for steers limit-fed rations with phosphorus concentrations ranging from .06 to .17 percent. Ruminal phosphorus concentration was increased with phosphorus intake at dietary concentrations below .12 percent for steers. This relationship was employed to estimate the ruminal availability of phosphorus from four phosphorus...

The objective of this study was to investigate the effects of inulin [as topinambur (Helianthus tuberosus L.) powder] on in vitro rumen fermentation of diets containing hay and pelleted concentrate for sheep (approximately 60:40%) in artificial rumen (the RUSITEC system). The RUSITEC system consisted of six fermentation vessels. Topinambur powder was added at levels of 0.2, 0.4, 0.6, 0.8 and 1....

In this study, nine male Dallagh sheep (initial weight 35±2 kg) were used in a replicated 3 × 3 latin square design experiment with three treatments and three 21-day periods (14 d adaptation, 7 d collection). Treatments were control (without addition) and two treatment diets containing 10% dry matter Glycyrrhiza glabra (licorice) and Urtica dioica (nettle) powder. The dry matt...