PURPOSE Aurora kinases play critical roles during mitosis in chromosome segregation and cell division. The aim of this study was to determine the preclinical profile of a novel, highly selective Aurora kinase inhibitor, PHA-680632, as a candidate for anticancer therapy. EXPERIMENTAL DESIGN The activity of PHA-680632 was assayed in a biochemical ATP competitive kinase assay. A wide panel of ce...

Together with other so-called "bio-plastics", Polyhydroxyalkanoates (PHAs) are expected to soon replace established polymers on the plastic market. As a prerequisite, optimized process design is needed to make PHAs attractive in terms of costs and quality. Nowadays, large-scale PHA production relies on discontinuous fed-batch cultivation in huge bioreactors. Such processes presuppose numerous s...

BACKGROUND Availability of antiretroviral therapies has transformed AIDS into a manageable chronic condition and improved well-being among people living with HIV/AIDS (PHA) in developed countries. In developing countries however, such transformations are yet to occur due to socio-economic, systemic and environmental constraint. This study examined the impact of social, economic, psychological a...

BACKGROUND Given its high surplus and low cost, glycerol has emerged as interesting carbon substrate for the synthesis of value-added chemicals. The soil bacterium Pseudomonas putida KT2440 can use glycerol to synthesize medium-chain-length poly(3-hydroxyalkanoates) (mcl-PHA), a class of biopolymers of industrial interest. Here, glycerol metabolism in P. putida KT2440 was studied on the level o...

The mitogen reactivity of peripheral blood and bone marrow cells from 5 patients with T-cell chronic (T-CLL) and 9 patients with T-ceII acute (T-ALL) Iymphoproliferative disease was investigated. Proliferative responsiveness to phytohemagglutinin (PHA) generally was found to be reduced or completely absent. Stimulation with PHA in the presence of the tumor promoter 1 2-0-tetradecanoyl phorbol-1...

The kinetics of ribosomal RNA transcription and processing were assessed in chronic lymphocytic leukemia (CLL) lymphocytes during the initial phases of their delayed response to phytohemagglutinin. When compared to cultures of normal lymphocytes, CLL cultures developed normal augmentations in 45S rRNA precursor transcription and cleavage after a 1 hr incubation with PHA. However, failure to con...

Peripheral blood lymphocytes from normal donors and patients with chronic lymphocytic leukemia, B-cell type, were purified into T, helper T, and suppressor T lymphocytes by fluorescence-activated cell sorting using OKT3, OKT4, and OKT8 monoclonal antibodies. The maximum response of the purified subpopulations to stimulation by phytohemagglutinin (PHA) was determined by measuring the production ...

XRD and EDX analyses confirmed the presence of Ag in all coatings deposited using electrochemical method and silver. The mean thickness coating was noted to be highest in EHA/AG group (102.20 4.20 um) followed by PHA (76.40 2.20 um). Mean coating thicknesses in other groups were: PHA/AgNO3 (76.20 1.29 um); EHA (32.98 2.50 um); EHA/ Ag/2-layers (30.70 2.40 um); EHA/AgNO3 (8.60 0.60 um). The aver...

= Abstract = Predictability for positive (or negativel of the three test protocols (RPHA only; RPHA-PHA; RPHA-PHA-CORABl, which were commonly used in assessing hepatitis B virus (HBVl infection, were evaluated with the reference method of RIA for HBsAg, anti-HBc and anti-HBs in 123 adults in Seoul. RPHA-PHA-CORAB and RPHA-PHA protocols were of great value in estimating prevalence, especially wh...

PURPOSE OF REVIEW This review provides background on the formation of the Partnership for a Healthier America (PHA), that was created in conjunction with the Let's Move! initiative, and an overview of its work to date. RECENT FINDINGS To encourage industry to offer and promote healthier options, PHA partners with the private sector. Principles that guide PHA partnerships include ensuring that...