The manganese peroxidase (MnP) of Phanerochaete chrysosporium supported Mn(II)-dependent, H(2)O(2)-independent lipid peroxidation, as shown by two findings: linolenic acid was peroxidized to give products that reacted with thiobarbituric acid, and linoleic acid was peroxidized to give hexanal. MnP also supported the slow oxidation of phenanthrene to 2,2'-diphenic acid in a reaction that require...

Ligninolytic white rot fungus, Phanerochaete chrysosporium, isolated from soil sample of petroleum refinery, was used for degradation of five polycyclic aromatic hydrocarbons (PAHs: acenaphthene, anthracene, phenanthrene, fluoranthene and pyrene), simultaneously and individually in sterile and unsterile soil. For maximum biodegradation, after 42 days of incubation, optimum conditions were pH 7....

The antifungal properties of some commercial plant waters obtained as side products in producing distilled oils were investigated. Paper disc samples were impregnated with various plant waters and exposed to wood decay fungi for 3 months in Petri dishes. Ten types of plant water (Laurus nobilis, Calluna vulgaris, Lavandula stoechas, Thymus vulgaris, Myrtus communis, Eucalyptus globulus, Mentha ...

Biodegradation of Orange II, Tropaeolin O, Congo Red, and Azure B in cultures of the white rot fungus, Phanerochaete chrysosporium, was demonstrated by decolarization of the culture medium, the extent of which was determined by monitoring the decrease in absorbance at or near the wavelength maximum for each dye. Metabolite formation was also monitored. Decolorization of these dyes was most exte...

The identity of the fungi associated with and responsible for wood decay could lead to the development of more environmentally benign wood preservative systems. In this study we have reconstructed the phylogenetic tree of a select group of basidiomycete fungi using nucleotide sequences of the internal transcribed spacer (ITS) region. We then compared this to trees generated from restriction fra...

The outer part of a tree, known as the bark, protects internal tissues from external conditions and attacks microorganisms. Its antifungal activities are due to presence chemicals in this bark. This study aimed at evaluating toxicity triterpenoids steroids bark Pinus merkusii against Phanerochaete chrysosporium (white-rot). were isolated n-hexane extract through column chromatography. Then, act...

In order to compare the degradation of PLA composites by different microorganisms, and reveal mechanism. Actinomyces israelii, Aspergillus niger, Trichoderma harzianum Phanerochaete chrysosporium were selected degrade composites. The weight loss, functional groups, thermal properties crystallinity before after analyzed. results indicated that A. israelii niger had strongest ability composites, ...

The US Department of Energy has assembled a high quality draft genome of Phanerochaete chrysosporium, a white rot Basidiomycete capable of completely degrading all major components of plant cell walls including cellulose, hemicellulose and lignin. Hundreds of sequences are predicted to encode extracellular enzymes including an impressive number of oxidative enzymes potentially involved in ligno...

Production of manganese-dependent peroxidase (MnP) by the white-rot fungus Phanerochaete chrysosporium BKMF-1767 (ATCC 24725) was monitored during growth in different media and growth conditions. The effect of some activators of MnP production, Mn, Tween 80, phenylmethylsulphonylfloride (PMSF), oxygen, temperature, pH, glycerol and nitrogen was studied. Supplementing the cultures with Tween 80 ...

A series of N-alkylated analogues of 1-deoxynojirimycin containing a fluorescent 10-chloro-9-anthracene group in the N-alkyl substituent were prepared. The anthracene group acted as a reporting group for protonation at the nitrogen in the iminosugar because an unprotonated amine was found to quench fluorescence by photoinduced electron transfer. The new compounds were found to inhibit bglucosid...