Simple and accurate spectrophotometric and HPLC method was developed for determination of Fexofenadine in tablets dosage form. The spectrophotometric method was developed by dissolving tablets in 1:1 Methanol to make solution of 10ppm giving absorbance at 220nm. The experimental conditions were optimized and Beers law was obeyed over the applicable concentration range. The application of HPLC p...

Chlorophyll analysis at high precision and accuracy is limited by the lack of suitable, commercially available internal standards for HPLC analysis. Here, the commercially available dye zinc-phthalocyanine is presented as a new internal standard to quantify chlorophylls in vegetable foods and to detect chlorophyll degradation products. The technique was applied to chlorophyll analysis of a sele...

New examples and sources of ghost peaks in reversed-phase gradient HPLC are described and related to previous publications to give a broad perspective of ghost peak problems. In one new example the ghost peak was found to be due to mixing problems caused by a period of non-delivery of the stronger "solvent B" in a stepped gradient system. In a second example the ghost peak was due to plasticize...

The HPLC/MS system, in which a monolithic silica capillary column is directly connected to an electronspray-ionization mass spectrometer, showed superior performance at high mobile phase linear velocity. A two-dimensional (2D) HPLC/MS system was established, using an ion-exchange particle-packed capillary column at the first dimension and a monolithic silica capillary column at the second dimen...

Previously, we developed a reversed-phase HPLC method compatible to high performance liquid chromatography diffuse reflectance Fourier-transform infrared (HPLC-FTIR) thermospray interface for the analysis of dyes. Dye separation achieved with a mixed-mode (SCX-ODS) column using a small gradient (90 to 80% water with acetic acid) and pH 3.25; 10 to 20% acetonitrile was considered to be suitable ...

Ultrafast reversed-phase high-performance liquid chromatographic (HPLC) separations are often needed for analyses related to combinatorial chemistry, studies in liquid chromatography-mass spectrometry, and other applications in which very rapid sample turnaround is paramount. Unfortunately, no consensus exists regarding the best column technology for optimally performing the desired rapid separ...

In accordance with the maximum residue limit of 100 micrograms kg-1 established by EU legislation, a simple and sensitive high-performance liquid chromatographic (HPLC) method was developed for the measurement of tylosin residues in pig tissues (fat, kidney, liver and muscle). Tylosin, a macrolide antibiotic, is extracted with water-methanol and cleaned-up by solid-phase extraction (SPE) on cat...

Rapid and simple method was developed for simultaneous determination of vitamins A and E contents in infant formula. Vitamins A and E were extracted by PDA-HPLC with reversed phase column using organic solvent, and their contents in Certified Reference Material (CRM) and infant formula were determined and compared with results of Food Standards Codex and AOAC method for evaluation of developed ...

A rapid, optimized, and sensitive reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed for determination of pentoxifylline in human plasma. The analyte was extracted from the plasma with dichloromethane after addition of 0.2 mL 1 M NaOH. HPLC separation was performed on a C18 analytical column (250 mm × 4 mm i.d.) with acetonitrile–water, 45:55 (v/v), as mo...

A reverse phase HPLC method with UV detection has been developed and validated in order to quantify plumbagin, the bioactive marker of the roots of P. indica and P. zeylanica. A quantitative HPTLC method was also developed using hexane: ethyl acetate (8:2) as the mobile phase. The plumbagin content in the roots were determined using both the methods. P. indica was found to contain significantly...