CELL CULTURE: Human neuroblastoma SH-SY5Y cells were cultured in a mixture of Ham’s F12 medium and Eagle’s minimal essential medium (1:1 mixture), supplemented with 10% (v/v) fetal calf serum and 1% nonessential amino acids. Cells were incubated at 37 °C in a humidified incubator gassed with 95% air and 5% CO2, passaged every 7 days and used for up to 20 passages. Cells were harvested in phosph...

Washed cells of Mycoplasma pneumoniae mixed with complete Freund's adjuvant were capable of producing lethal anaphylactic shock in mice when injected repeatedly into the foot pad. The causative agent is associated with horse serum contained in the culture medium but cannot be removed from mycoplasmal cells by repeated washing with phosphate buffered saline. Mycoplasmas grown in medium containin...

Significant inhibition of lymphoid tumor development was observed in X-irradiated strain C57BL mice treated with multiple injections of rat antiserum prepared by im munization of Wistar-Furth or (Wistar-Furth-BN) F, rats with histocompatible lymphomas induced by the radiation leukemia virus (RadLV). However, there was also signifi cant inhibition, relative to phosphate-buffered saline con trols...

One of the challenges faced by medical-device manufacturers is the use of short-term in-vitro data to evaluate the long-term in-vivo corrosion performance of implants. In this study, mechanically polished Nitinol wires were immersed in phosphate-buffered saline (PBS) at 37 °C for up to one week prior to performing cyclic polarization tests. Results are presented that show the effect of immersio...

Abbreviations: AM, acetoxymethylester; BSA, bovine serum albumin; DMEM, Dulbecco’s modified Eagle medium; FITC, fluorescein isothiocyanate; GM-CSF, granulocyte-macrophage colony-stimulating factor; HBSS, Hanks’ balanced salt solution; ICAM-1, intercellular adhesion molecule-1; LSC, laser scanning cytomety; NF-κB, nuclear factor-kappa B; PBS, phosphate buffered saline; PI, propidium iodide; PLP,...

We demonstrate the versatility of Al2O3-passivated Si nanowire devices ("nanoribbons") in the analysis of enzyme-substrate interactions via the monitoring of pH change. Our approach is shown to be effective through the detection of urea in phosphate buffered saline (PBS), and penicillinase in PBS and urine, at limits of detection of <200 μM and 0.02 units/mL, respectively. The ability to extrac...

Over the years, many ideas have been handed down from one technologist to another about what to do and what not to do when fixing cells and tissues. This set of experiments takes into account certain conditions involving the age of the prepared primary fixative, length of time in primary fixative, length of time en bloc stain is on tissue cultured cells and the effect of washing the cells befor...

Significant inhibition of lymphoid tumor development was observed in X-irradiated strain C57BL mice treated with multiple injections of rat antiserum prepared by im munization of Wistar-Furth or (Wistar-Furth-BN) F, rats with histocompatible lymphomas induced by the radiation leukemia virus (RadLV). However, there was also signifi cant inhibition, relative to phosphate-buffered saline con trols...

Nowadays antibiotics are used on a large scale in veterinary and human medicine to cure or prevent diseases. Some antibiotics injected into hatching eggs to eliminate pathogens and prevention of egg transmission of disease. Adverse effects of drugs have always been a major concern. There is scantly information available about the safety and pathological alterations of lincosamide-aminocyclitol ...

We describe the application of enzyme-linked immunomagnetic electrochemistry (ELIME) for the rapid detection of Escherichia coli O157:H7 in buffered apple juice. The ELIME technique entails sandwiching bacterial analyte between antibody-coated magnetic beads and an alkaline phosphatase-conjugated antibody. The beads (with or without bound bacteria) were localized onto the surface of magnetized ...