We evaluated the Clinistat Analyzer (Miles Inc., Diagnostics Division, Elkhart, IN) for measuring cholesterol, triglycerides, and high-density lipoprotein (HDL) cholesterol at three medical centers. The system, based on multilayer film technology, uses precalibrated, dry film reagent disks. Ten microliters of serum is applied to the dry film reagent disk in the test procedure. For HDL-cholester...

Phosphotungstic acid at low pH and colloidal iron hydroxide have been used to stain sialic acid residues at the surfaces of the transitional epithelium that covers the human urinary bladder. The staining of surfaces of normal epithelium was compared in the electron microscope to staining of surfaces of cells from noninvasive Grade I transitional cell carcinomas and from higher grades of invasiv...

We report on the performance of four HDL-cholesterol assays: a homogeneous method based on polyethylene glycol-modified enzymes/alpha-cyclodextrin sulfate (PEGME; Kyowa); a homogeneous method based on polyanion-polymer/detergent (PPD; Daiichi); the usual precipitation method with phosphotungstic acid/MgCl2 (PTA); and an ultracentrifugation (UC) procedure. The homogeneous HDL-cholesterol assays ...

Twelve adult rabbits of mixed stock weighing 1[5 to 2 5 kg. received intravenous injections of from 16 to 32 mg./kg. sodium bromoacetate. In three animals the dose was repeated on the following day. The nine survivors were killed for histological examination 3 to 21 days after injection. Two animals received combined injections of sodium bromoacetate (16 mg./kg.) and sodium iodoacetate (16 mg./...

Ultrastructural and cytochemical studies were carried out on sperm nucleus of the beetles, Coelomera lanio and Diabrotica speciosa. Nuclear development involves changes in the shape and in the degree of chromatin condensation, with specific aggregation patterns of DNA-histone complex occurring during this process. Lamellar and paracrystalline arrangements of the nuclear material were observed i...

This communication describes and evaluates an improved routine methodology for quantitating clinical proteinuria. Based on investigations of Piscator and of Savory et al., a modified Tsuchiya's reagent (ethanolic HCI-phosphotungstic acid) is used to precipitate proteins at 56 degrees C, followed by biuret spectrophotometry at 540 nm. The accuracy of the proposed procedure was assessed by compa...

Frayed cilia from Tetrahymena pyriformis have been examined in the electron microscope using both uranyl acetate and phosphotungstic acid as negative stains. Observations on the central microtubules show that one member of the pair exhibits two rows of short projections. These are about 18 nm long, and are spaced longitudinally at 16-nm intervals. In many specimens the projections are separated...

Chemical pretreatment can significantly improve the enzymatic hydrolysis efficiency of lignocellulosic biomass, thereby improving yield sugar materials for production cellulosic ethanol, but commonly used acid–base catalysts are difficult to recover and reuse. In this work, a combination method liquid hot water (LHW) phosphotungstic acid (PTA) was performed saccharification rice straw, we attem...