نتایج جستجو برای: physico chemical characterization

تعداد نتایج: 734653  

2017
Harisree P. Nair Helvin Vincent Rinu Madhu Puthusseri Sarita G. Bhat

Functional screening of a metagenomic library of marine sediment revealed an amylolytic clone BTM109. This report states the purification and characterization of a moderately halotolerant α-amylase, with more than 51% activity in 2.5 M NaCl. The molecular mass of purified protein was determined to be 55.7 kDa by MALDI-TOF MS. The optimum pH for enzyme activity was pH 7 and temperature for maxim...

Journal: :The Journal of antibiotics 1970
R K Sinha

During an antibiotic screening programme, a new biologically active complex was isolated from an unidentified Streptomyces, strain Ac6 5691}, which was obtained from a garden soil in Calcutta, India. This antibiotic complex was found to contain two water-soluble active fractions of which the major one was designated as boseimycin. This commuication describes the isolation, purification and char...

Journal: :European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V 2013
L Lemaire G Bastiat F Franconi N Lautram T Duong Thi Dan E Garcion P Saulnier J P Benoit

The assessment of tumor oxygenation is a crucial factor in cancer therapy and may be carried out using fluorine MRI once fluorine probes have been distributed within the tumor. However, the deposit of those highly fluorinated compounds often jeopardizes anatomical image quality and requires emulsification of the probes. Due to the high density and the high lipophilicity of perfluorocarbons, nan...

Journal: :Journal of structural biology 2010
Frank H Zucker Christine Stewart Jaclyn dela Rosa Jessica Kim Li Zhang Liren Xiao Jenni Ross Alberto J Napuli Natascha Mueller Lisa J Castaneda Stephen R Nakazawa Hewitt Tracy L Arakaki Eric T Larson Easwara Subramanian Christophe L M J Verlinde Erkang Fan Frederick S Buckner Wesley C Van Voorhis Ethan A Merritt Wim G J Hol

The great power of protein crystallography to reveal biological structure is often limited by the tremendous effort required to produce suitable crystals. A hybrid crystal growth predictive model is presented that combines both experimental and sequence-derived data from target proteins, including novel variables derived from physico-chemical characterization such as R(30), the ratio between a ...

Journal: :The Journal of antibiotics 1986
A Takahashi K Hotta N Saito M Morioka Y Okami H Umezawa

An actinomycete isolate designated as TS-1980 with multiple resistance to aminoglycoside antibiotics was found to produce novel antibiotics. The strain showed taxonomic features identical to the type strain of Nocardiopsis mutabilis except for the temperature range for growth and the utilization of mannitol and raffinose. Based on the capability of growing at lower temperature range, the strain...

Journal: :Nanoscale 2015
Santosh K Misra Tor W Jensen Dipanjan Pan

We describe for the first time a therapeutic strategy to target stem-like cancer cells via STAT-3 modulation using a nanomedicine approach. Niclocelle, a niclosamide loaded rigid core mixed micelle, was synthesized from a self-assembled well-defined amphiphilic diblock copolymer and an FDA-approved signal transducer and activator of transcription factor 3. Followed by a rigorous physico-chemica...

2014
Jan Stracke Thomas Emrich Petra Rueger Tilman Schlothauer Lothar Kling Alexander Knaupp Hubert Hertenberger Andreas Wolfert Christian Spick Wilma Lau Georg Drabner Ulrike Reiff Hans Koll Apollon Papadimitriou

Preserving the chemical and structural integrity of therapeutic antibodies during manufacturing and storage is a major challenge during pharmaceutical development. Oxidation of Fc methionines Met252 and Met428 is frequently observed, which leads to reduced affinity to FcRn and faster plasma clearance if present at high levels. Because oxidation occurs in both positions simultaneously, their ind...

Journal: :The Journal of antibiotics 1990
N R Krishna D M Miller T T Sakai

The assignments for the resonances in the 1H and 13C NMR spectra of mithramycin in aqueous solution have been determined by a combination of 2D NMR methods. Specific NOESY contacts observed between different moieties indicate that the drug assumes a compact conformation in aqueous solution. Fluorescence measurements are consistent with a compact structure for mithramycin in water and confirm th...

2013
F. Cucinotta F. Carniato L. Marchese

This contribution describes design, preparation and physico-chemical characterization of a new photostable hybrid antenna based on mesoporous SBA-15 silica. Concepts of host-guest chemistry are applied in such a way that one or more photoactive guest molecules are incorporated into the silica channels and on the outer surface, acting as energy harvesting and transferring units. The presented co...

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