Green, H., J. MacDougall, M. Tarnopolsky, and N. L. Melissa. Downregulation of Na1-K1-ATPase pumps in skeletal muscle with training in normobaric hypoxia. J. Appl. Physiol. 86(5): 1745–1748, 1999.—To investigate the effects of training in normoxia vs. training in normobaric hypoxia (fraction of inspired O2 5 20.9 vs. 13.5%, respectively) on the regulation of Na1-K1-ATPase pump concentration in ...

1. The ATPase activity of insect mitochondria has been investigated. A comparison was made to determine the distribution and nature of such activity in other isolated fractions of the house fly, Musca domestica L. 2. The ATPase in insect mitochondria is specific in that orthophosphate can be cleaved only from ATP. The Michaelis-Menten constant K(8) = 2.78 x 10(-3)M and V(max.) = 76 micrograms P...

Oligomycin sensitivity-conferring protein (OSCP) is a water-soluble subunit of bovine heart mitochondrial H(+)-ATPase (F1-F0). In order to investigate the requirement of OSCP for passive proton conductance through mitochondrial F0, OSCP-depleted membrane preparations were obtained by extracting purified F1-F0 complexes with 4.0 M urea. The residual complexes, referred to as UF0, were found to b...

Background—Oxidative stress and mitochondrial dysfunction are central mediators of cardiac dysfunction after ischemia/ reperfusion. ATP binding cassette mitochondrial erythroid (ABC-me; ABCB10; mABC2) is a mitochondrial transporter highly induced during erythroid differentiation and predominantly expressed in bone marrow, liver, and heart. Until now, ABC-me function in heart was unknown. Severa...

Nuclei from a mammary adenocarcinoma and liver indigenous to the same mouse, isolated by two different methods, were shown to contain adenosine triphosphatase (ATPase) activity. The distribution of ATPase activity in subcellular portions of the transplanted mammary tumor was ascertained and found to be highest in the mitochondrial and nuclear moieties. Comparison was made between the ATPase act...

BACKGROUND Plagiochin E (PLE) is an antifungal macrocyclic bis(bibenzyl) isolated from liverwort Marchantia polymorpha L. Its antifungal mechanism is unknown. To elucidate the mechanism of action, its effect on mitochondria function in Candida albicans was studied. METHODS We assayed the mitochondrial membrane potential (mtDeltapsi) using rhodamine 123, measured ATP level in mitochondria by H...

AIM To characterize the mitochondrial dysfunction in experimental cirrhosis and to study whether insulin-like growth factor-I (IGF- I) therapy (4 wk) is able to induce beneficial effects on damaged mitochondria leading to cellular protection. METHODS Wistar rats were divided into three groups: Control group, untreated cirrhotic rats and cirrhotic rats treated with IGF- I treatment (2 microg/1...

Mitochondrial H+-ATP synthase is required for cellular energy provision and for efficient execution of apoptosis. Almost one century ago, Otto Warburg proposed the hypothesis that mitochondrial function might be impaired in cancer cells. However, his hypothesis was never demonstrated in human carcinomas. In this study, we have analyzed the expression of the beta-catalytic subunit of the H+-ATP ...

all species in the genus lysiphlebus förster (hymenoptera: braconidae, aphidiinae) are solitary endoparasitoids of aphids, which are one of the most difficult and unknown taxonomic groups to identify. a pair of species-specific primers as a molecular marker was designed to identify the most abundant and important aphid parasitoid wasp, l. fabarum marshall, collected from guilan province (iran)....